User:Spitzer Gudrun/SV-Chamber Instructions: Difference between revisions
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== I Added sV Instructions to following pages == | |||
=== Same concentration for standard and small volume chamber === | |||
* [[Malate]] | |||
* [[Glutamate]] | |||
* [[Succinate]] | |||
* [[Octanoylcarnitine]] | |||
* [[ADP]] | |||
* [[ATP]] | |||
* [[Glucose]]: second paragraph is not in titrations list, leave it? | |||
* [[Glycerophosphate]] | |||
* [[Carnitine]] | |||
=== Different concentration for different chambers === | |||
==== Concentrations prepared separately ==== | |||
* [[Pyruvate]] | |||
* [[Ascorbate]] | |||
* [[TMPD]] | |||
* [[Cytochrome_c]] | |||
* [[KCN]] is the amount of stock solution and volume of aliquote ok? | |||
* [[Digitonin]] | |||
* [[Catalase]] why are different hamilton syringe sizes indicated on the table although the titration volume is the same for standard- and sV-chamber? | |||
* [[Hydrogen peroxide]]: preparation and titration are described on [[MiPNet14.13_Medium-MiR06#Re-oxygenation_with_H2O2_titrations]] | |||
==== Conc for sV-chamber by dilution ==== | |||
* [[CCCP]] | |||
* [[Rotenone]] | |||
* [[Malonate]] | |||
* [[Antimycin A]] | |||
* [[Myxothiazol]] | |||
* [[Sodium azide ]] | |||
* [[Carboxyatractyloside]] deleted higher concentration because not recommended any more | |||
* [[Oligomycin]] | |||
* [[FCCP]] | |||
* [[Ruthenium_red]] | |||
* [[SF6847]] | |||
== no sV-instructions == | |||
* [[Salicylhydroxamic acid]] because only used for algae. | |||
* [[Ap5A]] because not given by Luiza | |||
Latest revision as of 09:17, 17 May 2021
I Added sV Instructions to following pages
Same concentration for standard and small volume chamber
- Malate
- Glutamate
- Succinate
- Octanoylcarnitine
- ADP
- ATP
- Glucose: second paragraph is not in titrations list, leave it?
- Glycerophosphate
- Carnitine
Different concentration for different chambers
Concentrations prepared separately
- Pyruvate
- Ascorbate
- TMPD
- Cytochrome_c
- KCN is the amount of stock solution and volume of aliquote ok?
- Digitonin
- Catalase why are different hamilton syringe sizes indicated on the table although the titration volume is the same for standard- and sV-chamber?
- Hydrogen peroxide: preparation and titration are described on MiPNet14.13_Medium-MiR06#Re-oxygenation_with_H2O2_titrations
Conc for sV-chamber by dilution
- CCCP
- Rotenone
- Malonate
- Antimycin A
- Myxothiazol
- Sodium azide
- Carboxyatractyloside deleted higher concentration because not recommended any more
- Oligomycin
- FCCP
- Ruthenium_red
- SF6847
no sV-instructions
- Salicylhydroxamic acid because only used for algae.
- Ap5A because not given by Luiza
