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This page provides a simple browsing interface for finding entities described by a property and a named value. Other available search interfaces include the page property search, and the ask query builder.

List of results

• Illumination on/off  + (The illumination in both chambers is switched on/off.)
• Kelvin  + (The kelvin, symbol K, is the SI unit of thermodynamic temperature. It is defined by taking the fixed numerical value of the Boltzmann constant ''k'' to be 1.380 649 × 10⁻²³ when expressed in the unit J x^-1 K⁻¹.)
• Kilogram  + (The kilogram, symbol kg, is the SI unit of … The kilogram, symbol kg, is the SI unit of mass. It is defined by taking the fixed numerical value of the Planck constant ''h'' to be 6.626 070 15 × 10⁻³⁴ when expressed in the unit J s, which is equal to kg m² s⁻¹, where the meter and the second are defined in terms of ''c'' and Δ''ν''_Cs.he meter and the second are defined in terms of ''c'' and Δ''ν''_Cs.)
• Malate-aspartate shuttle  + (The malate-aspartate shuttle involves the … The malate-aspartate shuttle involves the glutamate-aspartate carrier and the 2-oxoglutarate carrier exchanging malate^2- for 2-oxoglutarate^2-. Cytosolic and mitochondrial malate dehydrogenase and transaminase complete the shuttle for the transport of cytosolic NADH into the mitochondrial matrix. It is most important in heart, liver and kidney.chondrial matrix. It is most important in heart, liver and kidney.)
• Mouse control: Mark  + (The mark mode is active by default, can be … The mark mode is active by default, can be selected in the menu or by [Ctrl+M]. If '''Mouse control: Mark''' is enabled, specific sections of the experiment can be marked in each plot. </br>Usually, marks are set on the plot for oxygen concentration for calibration, whereas marks on the plot for oxygen flux are set for exporting the median or average of flux to a table.</br></br>»More details: [[Marks - DatLab]].[Marks - DatLab]].)
• Wavelength range  + (The minimum and the maximum wavelengths ov … The minimum and the maximum wavelengths over which an [[absorbance spectrum]] is measured are described in terms of the [[wavelength range]]. It is determined mainly by the specifications of the [[spectrophotometer]] and the type of [[light source]] used, and the characteristic [[absorbance spectrum]] of the sample being investigated.[[absorbance spectrum]] of the sample being investigated.)
• Ergodynamics  + (The mission of '''ergodynamics''' is the r … The mission of '''ergodynamics''' is the revelation of relations of general validity. "''Thermodynamics deals with relationships between properties of systems at equilibrium and with differences in properties between various equilibrium states. It has nothing to do with time. Even so, it is one of the most powerful tools of physical chemistry''" [1]. '''Ergodynamics''' is the theory of exergy changes (from the Greek word 'erg' which means [[work]]). Ergodynamics includes the fundamental aspects of thermodynamics ('[[heat]]') and the thermodynamics of irreversible processes (TIP; nonequilibrium thermodynamics), and thus links thermodynamics to kinetics. In its most general scope, ergodynamics is the science of [[energy]] transformations. Classical thermodynamics includes [[open system]]s, yet as a main focus it describes [[closed system]]s. This is reflected in a nomenclature that is not easily applicable to the more general case of open systems [2]. At present, IUPAC recommendations [3] fall short of providing adequate guidelines for describing energy transformations in open systems.ng energy transformations in open systems.)
• Creatine kinase  + (The mitochondrial '''creatine kinase''', also known as phosphocreatine kinase (CPK), facilitates energy transport with [[creatine]] and [[phosphocreatine]] as diffusible intermediates.)
• Respiratory chain  + (The mitochondrial '''respiratory chain''' … The mitochondrial '''respiratory chain''' (RC) consists of enzyme complexes arranged to form a metabolic system of convergent pathways for [[oxidative phosphorylation]]. In a general sense, the RC includes (1) the [[electron transfer pathway]] (ET-pathway), with transporters for the exchange of reduced substrates across the inner mitochondrial membrane, enzymes in the matrix space (particularly dehydrogenases of the tricarboxylic acid cycle), inner membrane-bound electron transfer complexes, and (2) the inner membrane-bound enzymes of the [[phosphorylation system]].[[phosphorylation system]].)
• Mole  + (The mole [mol] is the SI base unit for the … The mole [mol] is the SI base unit for the [[amount |amount of substance]] of a system that contains 6.02214076·10²³ specified elementary entities (see [[Avogadro constant]]). The elementary entities must be specified and may be atoms, molecules, ions, electrons, other particles, or specified groups of such particles.ther particles, or specified groups of such particles.)
• Pyruvate carrier  + (The monocarboxylic acid [[pyruvate]]^- is exchanged electroneutrally for OH^- by the '''pyruvate carrier'''. H⁺/anion symport is equivalent to OH^-/anion antiport.)
• Drift  + (The most common cause of '''drift''' is variation in the intensity of the [[light source]]. The effect of this can be minimised by carrying out a [[balance]] at frequent intervals.)
• DatLab oxygen flux: performance and data analysis  + (The quality of the results are strongly affected by the performance and data analysis. Therefore, we provide guidelines for performing and evaluating respirometric assays.)
• Improvement score  + (The relative improvement score, ''RIS'', p … The relative improvement score, ''RIS'', provides a measure of improvement of a trait from a value measured at baseline, ''B'', to a value measured after treatment, ''T'', expressing the total improvement, ''T-B'', in relation to the theoretical scope of improvement and the level of the trait observed at baseline. '''RIS'' incorporates the concept of diminishing returns and consideres maintaining a high value of a trait as an improvement relative to the potential loss.mprovement relative to the potential loss.)
• Reproducibility crisis  + (The reproducibility crisis is alarming.< … The reproducibility crisis is alarming.¹ An experiment or study is ''reproducible'' or ''replicable'' when subsequent experiments confirm the results. This is [[research |re-search]]. However, we can define different types of reproducibility depending on the conditions that we use to replicate the previous work or in the information available. Our aim is to focus mostly on two different kinds²: '''1. Direct:''' is when we obtaining the same results using the same experimental conditions, materials, and methods as described in the original experiment. This would be the ideal reproducibility of an experiment. However, it requires a very accurate description of how the original experiment was performed. Some journals are trying to resolve the '''reproducibility crisis''' improving the rigor and the excellence on the reported methods and results (e.g. [https://www.cell.com/star-authors-guide STAR Methods in Cell Press]). '''2. Systematical:''' refers to obtaining the same results, but under different conditions; for example, using another cell line or mouse strain or humman study, or inhibiting a gene pharmacologically instead of genetically. This opens the door to subsequent studies to find the conditions under which an initial finding holds.udies to find the conditions under which an initial finding holds.)
• Second  + (The second, symbol s, is the SI unit of ti … The second, symbol s, is the SI unit of time. It is defined by taking the fixed numerical value of the caesium frequency ∆''ν''_Cs, the unperturbed ground-state hyperfine transition frequency of the caesium 133 atom, to be 9 192 631 770 when expressed in the unit Hz, which is equal to s⁻¹.ssed in the unit Hz, which is equal to s⁻¹.)
• Stoichiometric number  + (The sign of the '''stoichiometric number'' … The sign of the '''stoichiometric number''' ''ν''_X is determined by the nonspatial direction of the transformation (positive for products, negative for substrates), and the magnitude of ''ν''_X is determined by the stoichiometric form. For instance, ''ν''_A=-1 in the reaction 0 = -1 A + 2 B (-1 glucose converted to +2 lactate), but ''ν''_A=-1/6 in the reaction 0 = -1/6 A - 1 B + 1 C (-1/6 glucose and -1 O₂ converted to +1 H₂CO₃).1 B + 1 C (-1/6 glucose and -1 O₂ converted to +1 H₂CO₃).)
• Dithionite  + (The sodium salt of '''Dithionite''' Na< … The sodium salt of '''Dithionite''' Na₂S₂O₄ (Dit) is the 'zero oxygen solution powder' used for [[Oxygen calibration - DatLab |calibration of oxygen sensors]] at [[Zero calibration | zero oxygen concentration]], or for stepwise reduction of oxygen [[concentration]]s in [[MiPNet14.06 Instrumental O2 background |instrumental O₂ background tests]]. It is not recommended to use dithionite in experiments with biological samples or several multisensor approaches, for these see [[Setting the oxygen concentration]].[[Setting the oxygen concentration]].)
• Install Oroboros protocol package  + (The standard '''Instrumental and SUIT DL-P … The standard '''Instrumental and SUIT DL-Protocols''' package is automatically implemented with the simple DatLab programme installation. We recommend a 'clean install': rename your previous DatLab programme subdirectory (''e.g.'' C:\DatLab_OLD).</br>Updates and newly developed DL protocols can be simply downloaded by clicking on [Protocols]\Install Oroboros protocol package.tocols]\Install Oroboros protocol package.)
• Stirrer A on/off  + (The stirrer in chamber A is switched on/off.)
• Stirrer B on/off  + (The stirrer in chamber B is switched on/off.)
• Flux analysis - DatLab  + (The strategy of '''Flux analysis''' using … The strategy of '''Flux analysis''' using DatLab depends on the research question and the corresponding settings applied in DatLab when recording the data with the O2k. Usng [[MitoPedia: SUIT |SUIT protocols]], a sequence of respiratory steady-states is measured, marks are set, and numerical data are summarized in [[Mark statistics - DatLab|Mark statistics]] (F2). An AI approach is kept in mind when describing guidelines for evaluation of steady-states during data recording and analysis.states during data recording and analysis.)
• %  + (The symbol '''%''' indicates 'per cent' (per hundred). {''Quote''} The internationally recognized symbol % (per cent) may be used with the SI. When it is used, a space separates the number and the symbol %. {''end of Quote''}.)
• ≡  + (The symbol '''≡''' indicates (numerical) [[equivalence]], in contrast to = as the symbol for (physicochemical) [[equality]].)
• Open chamber  + (The term "open O2k-chamber" refers to a situation in which the liquid phase is allowed to equilibrate with a gas phase, but the stopper is partially inserted using the [[Stopper-Spacer]].)

• Extroduction  + (The term '''extroduction''' is ambiguous a … The term '''extroduction''' is ambiguous and needs introduction. An ''external'' extroduction aims at providing a specific exit that opens the door to the parent article. Once you popped up into the article box, there are various ''internal'' extroductions to push down by following hyperlinks to references, keywords, supplementary material, and to the external extroduction. Once you have pushed one level down, there may be hyperlinks to push down further ([[Hofstadter 1979 Harvester Press |Hofstadter 1979]]). One needs to keep track of the links in a nested network of open tabs, to pop up all the way back for returning to the initial reference level. returning to the initial reference level.)
• Incident light  + (The term '''incident light''' is used for a beam of light falling upon a surface.)
• Isomorphic  + (The term '''isomorphic''' refers to quanti … The term '''isomorphic''' refers to quantities which have [https://www.merriam-webster.com/dictionary/isomorphic ''identical or similar form, shape, or structure'']. In mathematics, an isomorphism defines a [https://www.merriam-webster.com/dictionary/isomorphism ''one-to-one correspondence between two mathematical sets'']. In [[ergodynamics]], isomorphic quantities are defined by equations of identical form. If isomorphic quantities are not expressed in identical units, then these quantities are expressed in different formats which can be converted to identical untis. Example: electric force [V=J/C] and chemical force [Jol=J/mol] are ismorphic [[force]]s; the electrical format [J/C] can be converted to the chemical format [J/mol] by the [[Faraday constant]]. Units not only give meaning to the numerical value of a quantity, but units provide also an abbreviated common language to communicate and compare isomorphic quantities. In irreversible thermodynamics, isomorphic forces are referred to as ''generalized'' forces.are referred to as ''generalized'' forces.)
• System  + (The term '''system''' has a variety of mea … The term '''system''' has a variety of meanings and dictionary definitions in different contexts, ''e.g.'', the [[International System of Units]] (SI), MKSA system, data management system, biological or mechanical system, redox system, [[Electron transfer system]], loosely or completely coupled system, instrumental system. In thermodynamics and [[ergodynamics]], the '''system''' is considered as an experimental system (experimental chamber), separated from the environment as an isolated, adiabatic, closed, or open system. {''Quote'' } The internal domain of any system is separated from the external domain (the surroundings) by a boundary. In theory, energy transformations outside the system can be ignored when describing the system. The surroundings are merely considered as a source or sink for quantities transferred across the system boundary. According to the transfer properties of the boundary, three types of thermodynamic systems are distinguished. (''1'') The boundaries of '''''isolated systems''''' are impermeable for all forms of [[energy]] and matter. Isolated systems do not interact with the surroundings. Strictly, therefore, internal changes of isolated systems cannot be observed from outside since any observation requires interaction. (''2'') The boundaries of '''''closed systems''''' are permeable for [[heat]] and [[work]], but impermeable for [[matter]]. A limiting case is electrons which cross the system boundary when work is exchanged in the form of electric energy [''added'': and light]. The volume of a closed system may be variable. (''3'') The boundaries of '''''open systems''''' allow for the transfer of heat, work and matter. Changes of isolated systems have exclusively internal origins, whereas changes of closed and open systems can be partitioned according to internal and external sources. Production and destruction of a quantity within the system are ''internal'' changes, whereas changes of heat, work and matter due to transfer across the system boundaries are labelled ''extenal''. (External) transfer is thus contrasted with (internal) production or destruction. {''end of Quote'': [[Gnaiger 1993 Pure Appl Chem]]}</br></br>A system may be treated as a black box. In the analysis of [[Continuous system|continuous]] or [[Discontinuous system |discontinuous system]]s, however, information is implied on the internal structure of the system.d on the internal structure of the system.)
• Hydrogen ion  + (The terms '''hydrogen ion''' H^{+ … The terms '''hydrogen ion''' H⁺ and [[proton]], p or p⁺, are used synonymously in chemistry. A hydrogen ion is a positively charged molecule. In particle physics, however, a proton is a submolecular and subatomic particle with a positive electric charge. The H⁺ ion has no electrons and is a bare charge with only about 1/64 000 of the radius of a hydrogen atom. Free H⁺ is extremely reactive, with an extremely short lifetime in aqueous solutions. There H⁺ forms the hydronium ion H₃O⁺, which in turn is further solvated by water molecules in clusters such as H₅O₂⁺ and H₉O₄⁺. The transfer of H⁺ in an acid–base reaction is referred to as ''proton transfer''. The acid is the H⁺ donor and the base is the H⁺ acceptor.lt;sup>+}. The transfer of H⁺ in an acid–base reaction is referred to as ''proton transfer''. The acid is the H⁺ donor and the base is the H⁺ acceptor.)
• Proton  + (The terms '''proton''' p and [[hydrogen ion]] … The terms '''proton''' p and [[hydrogen ion]] H⁺ are used synonymously in chemistry. In particle physics, a proton is a subatomic particle with a positive electric charge. Protons and neutrons are collectively referred to as ''nucleons''. The proton is a bare charge with only about 1/64 000 of the radius of a hydrogen atom, and so the free proton is extremely reactive chemically. Therefore, the free proton has an extremely short lifetime in aqueous solutions where it forms the [[hydronium ion]], H₃O⁺, which in turn is further solvated by water molecules in clusters such as H₅O₂⁺ and H₉O₄⁺.;sub>5</sub>O₂⁺ and H₉O₄⁺.)
• SI prefixes  + (There are 20 '''SI prefixes''' defined to represent multiples and submiltiples of SI units.)
• Sides  + (There are many '''sides''' of the term 'si … There are many '''sides''' of the term 'side' in our language system. Inside and outside are the sides that are separated by the system boundaries of an experimental [[system]]. + and - are the two sides of numbers separated by 0. Pages in books have opposite sides or front sides versus backsides. Many fundamental terms have opposite sides of the meaning, thus spanning the entire message in the space between their apparently contrasting sides, and transforming the paradox as a perspective into the unified whole, the full, the complete. On the other side, such fundamental terms are fully understood only after ''realization'' of the opposite sides of their meaning — treasures discovered in the etymological origins of the word. It makes sense to open all our senses to comprehend the bright side and the dark side of things. Whereas the student sais "I see a black sheep", Zen decides "You see, that one side of the sheep is black". This is the message to consider both sides before choosing sides, besides overcoming a one-sided point of view. Don't rock side-to-side, but get immersed deeply inside things to see the upsides and downsides of every thing or anything, and more so of nothing. Inside is the insight, for insiders and outsiders of the feedback loop of an [[Ouroboros]].Ouroboros]].)
• DatLab and SUIT protocols  + (This is a brief summary of steps to be tak … This is a brief summary of steps to be taken for performing a high-resolution respirometry experiment with '''[[SUIT protocols]]''' using the OROBOROS [[Oroboros O2k]] and '''[[DatLab]]''' software. (1) Search for a specific [[SUIT protocol name]] (go to [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT]]). The list of MitoPedia SUIT protocols can be sorted by [[categories of SUIT protocols]] (sorting by SUIT protocol name), which is listed as the 'abbreviation' of the SUIT protocol name. (2) Copy the template for [[Mark names]] into your DatLab subdirectory: DatLab\APPDATA\MTEMPLAT. (3) Copy the [[DatLab-Analysis templates |DatLab-Analysis template]] for this SUIT protocol. (4) Follow the link to the corresponding publication or MiPNet communication, where the pdf file describing the SUIT protocol is available. (5) A DatLab demo file may be available providing an experimental example. After each sequential titration, a mark is set on the plot for flux or flow. After having set all marks, pull down the 'Mark names' menu, select the corresponding SUIT protocol for mark names, and rename all marks. The Mark names template also provides standard values of the titration volume preceding each mark. (6) Go to 'Mark statistics' [F2], copy to clipboard, and paste into the sample tab in the DatLab-Analysis template.</br></br>: Example:</br>:* SUIT protocol name: [[SUIT-011]]</br>:* Mark names in DatLab: 1GM;2D;2c;3S;4U;5Rot-</br>:* DatLab-Analysis template: SUIT_NS(GM)01.xlsx</br>:* MiPNet communciation: [[MiPNet12.23 FibreRespiration]]</br>:* DatLab demo file: MiPNet12.23 FibreRespiration.DLDemo file: MiPNet12.23 FibreRespiration.DLD)
• Beer-Lambert law  + (This law states that the [[transmittance]] … This law states that the [[transmittance]] (''T'') of light though a sample is given by:</br>''T'' = e^-''εbc'', where ''ε'' is the molar [[extinction coefficient]], ''b'' is the pathlength of the light through the cuvette (in mm) and ''c'' is the concentration of the pigment in the sample (in mM). Transforming this equation, it can be seen that the [[absorbance]] of light (''A'') is simply given by ''A'' = ''εbc''.absorbance]] of light (''A'') is simply given by ''A'' = ''εbc''.)
• Least squares method  + (This method makes use of all of the data p … This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spectrum of known concentration using a '''least squares method''' to match the measured spectrum with the reference spectrum. The technique results in improved accuracy compared with the use of only a few characteristic wavelengths. of only a few characteristic wavelengths.)
• Paywall journalism  + (Though often defined from the individual r … Though often defined from the individual reader's perspective, a paywall can also apply to an institution (such as a library) or the author. '''Paywall journalism''' is the opposite of [[Open Access]]. [[Open Science]] does not accept paywalls with the argument, that the public pays for governmentally funded research, hence research funded by public grants should be published with open access for the public without paywalls. Paywalls are most frequently defined from the perspective of the individual reader, who has to pay for an article or pay a journal subscription as a requisite for obtaining full access to the information that is otherwise hidden behind the paywall ('''reader-paywall journal'''). From the perspective of the authors, however, an '''author-paywall journal''' is defined as any journal which requests publication charges or page charges from the authors for publishing the manuscript Open Access or publishing it at all. Similarly, an '''institutional-paywall journal''' charges an institution – typically university libraries – for granting open access to the members of this institution. As long as paywall journalism prevails in science, at least '''paywall transparence''' should be required, to declare for each publication not only the reader-paywall costs but provide the full information on the author-paywall and institutional-paywall expenses.aywall and institutional-paywall expenses.)
• O2k signals and output  + (Three electronic '''channel types''' are a … Three electronic '''channel types''' are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]. All channels are available twofold (dual-data), for O2k-Chambers A (left) and B (right), based on numerical signals sent at a fixed data sampling time interval (default: 2 s; range 0.2 s to >10 s).rval (default: 2 s; range 0.2 s to >10 s).)
• Triethyltin bromide  + (Triethyltin bromide (TET) is a lipophilic [1] inhibitor of the mitochondrial [[ATP synthase]] [2] which is used to induce [[LEAK state]] in [[living cells]] of ''Saccharomyces cerevisiae''.)
• POS calibration - static  + (Two-point calibration of the polarographic oxygen sensor, comprising [[Air calibration]] and [[Zero calibration]]. See also [[POS calibration - dynamic]].)
• Unspecific binding of TPP+  + (Unspecific binding of the probe molecule T … Unspecific binding of the probe molecule TPP⁺ in the matrix phase of mitochondria is taken into account as a correction for measurement of the [[mitochondrial membrane potential]]. External unspecific binding is the binding outside of the inner mt-membrane or on the outer side of the inner mt-membrane, in contrast to internal unspecific binding.-membrane, in contrast to internal unspecific binding.)
• SUITbrowser  + (Use the '''SUITbrowser''' to find the subs … Use the '''SUITbrowser''' to find the substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocol most suitable for addressing your research questions.</br></br> <big><big>Open the SUITbrowser: http://suitbrowser.oroboros.at/</big></big></br></br></br>[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]w.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)])
• Getting started - DatLab  + (Users have to enter their user details the first time they use DatLab 8 on a specific computer. As well, entering some basic settings is required when connecting DatLab 8 with an O2k for the first time.)
• Valinomycin  + (Valinomycin catalyzes electrogenic K⁺ transport down the electrochemical transmembrane gradient (150 ng^.mg^-1 protein).)
• Smoothing  + (Various methods of '''smoothing''' can be … Various methods of '''smoothing''' can be applied to improve the [[signal-to-noise ratio]]. For instance, data points recorded over time [s] or over a range of wavelengths [nm] can be smoothed by averaging ''n'' data points per interval. Then the average of the ''n'' points per smoothing interval can be taken for each successively recorded data point across the time range or range of the spectrum to give a ''n''-point moving average smoothing. This method decreases the [[noise]] of the signal, but clearly reduces the time or wavelength [[resolution]]. More advanced methods of smoothing are applied to retain a higher [[time resolution]] or wavelength resolution.[[time resolution]] or wavelength resolution.)
• Hydrogenion flux  + (Volume-specific '''hydrogenion flux''' or … Volume-specific '''hydrogenion flux''' or H⁺ flux is measured in a closed system as the time derivative of H⁺ concentration, expressed in units [pmol·s^-1·mL^-1]. H⁺ flux can be measured in an open system at steady state, when any acidification of the medium is compensated by external supply of an equivalent amount of base. The extracellular acidification rate (ECAR) is the change of pH in the incubation medium over time, which is zero at steady state. Volume-specific H⁺ flux is comparable to volume-specific [[oxygen flux]] [pmol·s^-1·mL^-1], which is the (negative) time derivative of oxygen concentration measured in a closed system, corrected for instrumental and chemical background.</br></br>[[pH]] is the negative logarithm of hydrogen ion activity. Therefore, ECAR is of interest in relation to acidification issues in the incubation buffer or culture medium. The physiologically relevant metabolic H⁺ flux, however, must not be confused with ECAR.e incubation buffer or culture medium. The physiologically relevant metabolic H⁺ flux, however, must not be confused with ECAR.)
• Different O2 fluxes in left and right chamber  + (What are potential causes for '''different O₂ fluxes in the left and right chamber'''?)
• Transmittance  + (When light enter a sample, '''transmittance''' (''T'') is the fraction of the intensity (''I'') of the light emerging from the sample compared with the incident light intensity (''I''_''0''): ''T'' = ''I''/''I''_''0''.)
• Absorption  + (When light enters a sample and emerges wit … When light enters a sample and emerges with an intensity (''I''), '''absorption''' (''Abs'') is the fraction of the light absorbed by the sample compared with the [[incident light]] intensity (''I''_''0''): ''Abs'' = 1-''I''/''I''_''0''. Absorption can also be expressed as ''Abs'' = 1-''T'', where ''T'' is the [[transmittance]].[[transmittance]].)
• Absorbance spectrum  + (When light enters a sample, the amount of … When light enters a sample, the amount of light that it absorbs is dependent upon the wavelength of the incident light. The '''absorbance spectrum''' is the curve derived by plotting the measured [[absorbance]] against the wavelength of the light emerging from the sample over a given [[wavelength range]]. An [[absorbance spectrum]] may be characterised by peaks and troughs (absorbance maxima and minima) that can be used to identify, and sometimes quantify, different absorbing substances present in a sample. absorbing substances present in a sample.)