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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country City Contact Armenia Yerevan Trchounian K Austria Innsbruck Gnaiger Erich Austria Innsbruck Cardoso Luiza HD Austria Innsbruck Garcia-Souza Luiz F Austria Innsbruck Doerrier Carolina Austria Innsbruck Karabatsiakis Alexander Austria Innsbruck Fischer Christine Austria Innsbruck Meszaros Andras T Austria Innsbruck Hoppel Florian Austria Innsbruck Torres-Quesada Omar Belgium Namur Arnould Thierry Belgium Liege Votion Dominique-Marie Belgium Namur Detraux Damien Belgium Namur Renard P Belgium Leuven Bird Matthew Bosnia and Herzegovina Tuzla Jusic Amela Bosnia and Herzegovina Tuzla Hajrulahovic Anesa Brazil Sao Paulo Alencar Mayke Bezerra Brazil São Paulo Crispim Marcell Brazil Sao Paulo Verdaguer Ignasi Bofill Brazil Sao Paulo Dominguez-Amorocho OA Croatia Zagreb Rubelj Ivica Czech Republic Prague Mracek Tomas Czech Republic Vestec Zobalova Renata Czech Republic Pilsen Kripnerova M Czech Republic Prague Elkalaf Moustafa Czech Republic Pilsen Markova Michaela Czech Republic Hradec Kralove Vernerova Andrea Czech Republic Hradec Kralove Maseko Tumisang Edward Czech Republic Prague Fisar Zdenek Czech Republic Pilsen Kuncova J Czech Republic Prague 4 Bajzikova Martina Czech Republic Prague Pecina Petr Czech Republic Prague Hansikova Hana Czech Republic and Australia Prague and Southport Neuzil Jiri Czech rep Hradec Kralove Falaye T Denmark Hvidovre Nehlin Jan O Denmark Aarhus Rattan Suresh IS Denmark Malov Hey-Mogensen M Denmark Copenhagen Desler C Denmark Copenhagen Rasmussen LJ Denmark Copenhagen N Mortensen OH Egypt Cairo Ali Sameh S Egypt Cairo Abdel-Rahman Engy Ali Estonia Tallinn Truu Laura Estonia Tallinn Klepinina Lyudmila Estonia Tartu Grellier T Finland Jyvaeskylae Lehti Maarit Finland Turku Stier Antoine Finland Helsinki Pirinen E France Paris Petit Patrice X France Bordeaux cedex Mourier A France Bron Rizwan Alam M Germany Leipzig Ost Mario Germany Mainz Vujacic-Mirski Ksenija Germany Regensburg Renner-Sattler Kathrin Germany Duesseldorf Piel Sarah Germany Freising Keppner Gloria Germany Magdeburg Vieira Ligo Teixeira Camila Germany Giessen Eckert Gunter P Germany Mainz Kalinovic S Germany Munich Jastroch M Germany Freising-Weihenstephan Musiol Eva Germany Biberach an der Riß Nold V Germany Hamburg Singer Dominique Germany Ulm Hartmann C Germany Freising Schoettl T Germany Duesseldorf Eckers A Germany Ulm Calzia Enrico Germany Ulm Schaefer PM Germany Neuherberg Borchard Sabine Germany Ulm Kolassa Iris Germany Gießen Silaidos Carmina Germany Düsseldorf Prigione Alessandro Germany Freising Klingenspor Martin Germany Freising Fromme Tobias Greece Athens Trougakos Ioannis P Hungary Budapest Tretter Laszlo Hungary Budapest Komlodi Timea Hungary Debrecen Verebne Tar K Ireland Dublin Karavyraki Marilena Ireland Dublin Porter Richard K Ireland Sligo Murphy J Israel Rishon Le Zion Hachmo Yafit Israel Zrifin Sova Marina Israel Jerusalem Saada Reisch Ann Italy Bolzano/Bozen Volani Chiara Italy Bologna Paterlini S Italy Ancona Battino Maurizio Italy Padova Fernandez-Vizarra Erika Italy Bologna Porcelli AM Italy Bolzano Castelo Rueda Maria Paulina Italy Bologna Gasparre G Italy Rome Scatena Roberto Italy Milano Bottoni P Italy Verona Calabria Elisa Italy Bologna Genova Maria Luisa Italy Bolzano Pichler Irene Japan Sapporo Shirakawa R Latvia Riga Makarova Elina Latvia Riga Kidere Dita Malaysia Kuala Lumpur Abdul Karim Norwahidah Malta Msida Vella Joanna Netherlands Groningen Rots MG Netherlands Wageningen Keijer Jaap Netherlands Nijmegen Koopman Werner JH Norway Oslo Nikolic N Norway Oslo Rustan AC Norway Bergen Tronstad Karl Johan Norway Oslo Lund Jenny Norway Oslo Eide L Palestine Ramallah Stiban Johnny Poland Lodz Siewiera Karolina Poland Warsaw Wasilewski M Poland Krakow Kaczara P Poland Poznan Michalak Slawomir Poland Poznan Swiniuch Daria Poland Warsaw Kampa Rafal Pawel Poland Warsaw Sek Aleksandra Poland Warsaw Dymkowska Dorota Poland Poznan Rybacka-Mossakowska Joanna Poland Lodz Watala Cezary Poland Lodz Labieniec-Watala Magdalena Poland Poznań Ludziejewska A Portugal Porto Moreira Bruno P Portugal Cantanhede Bento Guida Jose Freitas Portugal Porto Oliveira Pedro Fontes Portugal Coimbra Palmeira Carlos Portugal Covilhã Dias Tania R Portugal Coimbra Pereira Susana P Portugal Porto Bernardino RL Portugal Porto Alves Marco G Portugal Cantanhede Pinho Sonia A Portugal Porto Beleza Jorge Portugal Catanhede Pereira da Silva Grilo da Silva Filomena Portugal Porto Carrageta D Portugal Porto Magalhaes Jose Portugal Porto Martins Ana Dias Portugal Porto Crisostomo Luis Portugal Coimbra Carvalho Eugenia Republic of Korea Daejeon Park SH Republic of Korea Yuseong-gu Daejeon Quang Don T Republic of Serbia Belgrade Pavlovic Kasja Republic of Serbia Belgrade Krako Jakovljevic Nina Romania Timisoara Betiu AM Romania Timisoara Avram Vlad F Romania Timisoara Danila Maria-Daniela Romania Timisoara Muntean Danina M Romania Timisoara Lelcu Theia Romania Timisoara Lighezan R Russia Moscow Itkis YS Russia Moscow Tsygankova P Russia Moscow Krylova T Saudi Arabia Giza Kotb IA Serbia Belgrad Markovic Ivanka Serbia Belgrade Misirlic-Dencic S Serbia Belgrade Ivanovic Tijana Serbia Belgrade Jeremic M Serbia Belgrade Isakovic A Serbia Belgrade Vukojevic M Slovakia Bratislava Ukropcova B Slovakia Bratislava Ukropec J Slovakia Bratislava Maderova D Slovakia Bratislava Nemec Michal Slovakia Bratislava Sumbalova Zuzana Slovakia Košice Cizmarova Beata Slovenia Ljubljana Kopitar-Jerala Natasa Slovenia Ljubljana Pirkmajer Sergej Slovenia Ljubljana Mars T Spain Madrid Cano-Abad MF Spain Ourense Reboredo-Rodriguez Patricia Spain A Coruña Blanco FJ Spain Lleida Gonzalo Hugo Spain Madrid Monsalve Maria Spain Madrid Lopez MG Spain Valencia Borras Consuelo Spain Lleida Nogueras L Spain Madrid Cadenas S Spain Barcelona Dahdah Norma Ramon Spain Valencia Fuertes Agudo Marina Spain Barcelona Gonzalez Casacuberta I Spain Sevilla Muntane Jordi Spain Valencia Casado Pinna Marta Spain Granada Quiles JL Spain Sevilla Bustos Matilde Spain Salamanca Almeida Angeles Spain Barcelona Tura Ceide O Spain Barcelona Juarez D Spain Barcelona Hyrossova Petra Spain Barcelona Zorzano Antonio Spain Barcelona Arago Marc Spain A Coruña Mayan MD Spain Madrid De los Rios C Spain Armilla (Granada) Fernandez-Ortiz Marisol Spain Barcelona Smolders V Spain Barcelona Gama Perez Pau Spain Barcelona Gil J Spain Valencia Victor Victor Manuel Spain Armilla, Granada Escames Germaine Sweden Lund Simon Serrano Sonia Sweden Lund Leffler M Sweden Lund Westerlund E Sweden Lund Abliva Sweden Lund Karlsson M Sweden Linköping Fontes-Oliveira CC Sweden Lund Elmer Eskil Sweden Lund Chamkha Imen Sweden Södertälje Alfaro-Moreno E Sweden, SE Lund Aasander Frostner Eleonor Switzerland Lausanne Zanou Nadege Switzerland Geneva Martinou JC The Netherlands BT Amsterdam Wuest Rob CI Turkey Ankara Engin Ayse Basak Turkey Ankara Tuncay Erkan Turkey Istanbul Baykal AT Turkey Tekirdag Aral Cenk Turkey Izmir Aydin HH USA St Louis Welch R USA Fayetteville Rao RR USA Philadelphia Kilbaugh Todd USA Philadelphia Starr Jonathan USA Orlando Coen Paul M USA Baton Rouge Irving Brian A USA Philadelphia Janowska Joanna USA Fayetteville Iyer Shilpa USA Iowa City Buettner Garry R United Kingdom Stoke-on-Trent McStay Gavin P United Kingdom Oxford Makrecka-Kuka Marina United Kingdom Leicester Moisoi Nicoleta United Kingdom Birmingham Thakker A United Kingdom Birmingham Joanisse S United Kingdom Brighton Moore Anthony L Uruguay Montevideo Radi R
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
