Difference between revisions of "MitoPediaAlert2020 Archive"
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== Jul 2020 == | == Jul 2020 == | ||
* '''Jul-23 Th''' | |||
* '''Jul- | :{| | ||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | |||
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' | |||
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br> | |||
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''« | |||
|} | |||
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* '''Jul-16 Th''' | |||
:{| | :{| | ||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | |||
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br> | |||
- | Using different Fluorescence-Sensors and [[Filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential]], [[Calcium]], and numerous other applications open for O2k-user innovation.<br> | ||
- | - »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors | Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module | Setup of the O2k-Fluo LED2-Module]]'''« | ||
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- | |||
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* '''Jul- | * '''Jul-02 Th''' | ||
:{| | :{| | ||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | ||
| ''' | | '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br> | ||
- loss of oxidative capacity during the time course of the respirometric assay,<br> | |||
- | - insufficient [[Uncoupler|uncoupler]] concentrations,<br> | ||
- over titration of an uncoupler, which inhibits ET capacity,<br> | |||
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity. | |||
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« | |||
|} | |} | ||
Revision as of 08:15, 20 July 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Jul 2020
- Jul-23 Th
Do you know how to assess the integrity of the mitochondrial outer membrane in mitochondrial preparations? It is possible to do so using exogenously added cytochrome c:
- »Cytochrome c control factor«
- Jul-16 Th
Do you know how to select the proper Fluorescence-Sensor and filter set?
Using different Fluorescence-Sensors and Filters, the O2k-Fluo Smart-Module allows for measurement of Hydrogen peroxide, ATP, Mitochondrial membrane potential, Calcium, and numerous other applications open for O2k-user innovation.
- » Selecting the Smart Fluo-Sensor« and » Setup of the O2k-Fluo LED2-Module«
- Jul-09 Th
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?
- Jul-02 Th
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity. Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«