Difference between revisions of "SF6847"
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|description='''SF 6847''' (C<sub>18</sub>H<sub>22</sub>N<sub>2O</sub>), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent [[uncoupler]] of [[oxidative phosphorylation]], being used in the nM range. Like all uncouplers, SF 6847 concentrations must be titrated carefully to evaluated the [[Uncoupler#Optimum_uncoupler_concentration|optimum concentration]] for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations. | |description='''SF 6847''' (C<sub>18</sub>H<sub>22</sub>N<sub>2O</sub>), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent [[uncoupler]] of [[oxidative phosphorylation]], being used in the nM range. Like all uncouplers, SF 6847 concentrations must be titrated carefully to evaluated the [[Uncoupler#Optimum_uncoupler_concentration|optimum concentration]] for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations. | ||
|info=[[Chinopoulos 2014 Methods Enzymol]], [https://www.ncbi.nlm.nih.gov/pubmed/6498178 Terada 1984 Biochim Biophys Acta] | |info=[[Chinopoulos 2014 Methods Enzymol]], [https://www.ncbi.nlm.nih.gov/pubmed/6498178 Terada 1984 Biochim Biophys Acta] | ||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
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:::: '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. | :::: '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. | ||
{{Keywords: Uncoupling}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Uncoupler | |||
}} |
Revision as of 09:13, 19 August 2020
Description
SF 6847 (C18H22N2O), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent uncoupler of oxidative phosphorylation, being used in the nM range. Like all uncouplers, SF 6847 concentrations must be titrated carefully to evaluated the optimum concentration for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.
Abbreviation: SF6847
Reference: Chinopoulos 2014 Methods Enzymol, Terada 1984 Biochim Biophys Acta
Application in HRR
Preparation
- SF 6847/tyrphostin A9/malonoben (C18H22N2O, [[3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenyl]methylene]propanedinitrile; Sigma T182, 5 mg: FW = 282.38. Alternative source: Enzo, BML-EI215.
- Caution: Toxic!
- Preparation of 50 mM stock solution (dissolved in 99.9% ethanol):
- To 5 mg in a glass vial: dissolve with 354.1 ยตL ethanol.
- Store in glass vials at -20 ยฐC.
- Preparation of 50 mM stock solution (dissolved in 99.9% ethanol):
- Preparation of 500 ยตM stock solution (dissolved in 99.9% ethanol):
- Add 990 ยตL ethanol and 10 ยตL of the 50 mM stock solution to a glass vial.
- Store in glass vials at -20 ยฐC.
- Preparation of 500 ยตM stock solution (dissolved in 99.9% ethanol):
- Preparation of 50 ยตM stock solution (dissolved in 99.9% ethanol):
- Add 900 ยตL ethanol and 100 ยตL of the 500 ยตM stock solution to a glass vial.
- Divide into 0.1 ml portions, or store in a glass vial and use subsamples during experiments to avoid contamination of the storage solution.
- Store in glass vials at -20 ยฐC.
- Preparation of 50 ยตM stock solution (dissolved in 99.9% ethanol):
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 ยตl steps using a 10 ยตl syringe (2 ml O2k-Chamber).
- Final concentration: 25 nM steps.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- ยป Artefacts by single dose uncoupling
- ยป ATP synthase
- ยป CCCP
- ยป Coupling-control protocol
- ยป DNP
- ยป Dyscoupled respiration
- ยป FCCP
- ยป Is respiration uncoupled - noncoupled - dyscoupled?
- ยป Noncoupled respiration: Discussion
- ยป Uncoupler
- ยป Uncoupled respiration - see ยป Noncoupled respiration
- ยป Uncoupling proteins
- ยป Uncoupling protein 1
- ยป Uncoupler titrations - Optimum uncoupler concentration
- Specific
- Respiratory states and control ratios
- ยป Biochemical coupling efficiency
- ยป Coupling-control state
- ยป Electron-transfer-pathway state
- ยป Electron-transfer pathway
- ET capacity
- ยป E-L coupling efficiency
- ยป Flux control efficiency
- ยป Flux control ratio
- ยป LEAK-control ratio
- ยป LEAK respiration
- ยป Noncoupled respiration
- ยป OXPHOS
- ยป OXPHOS capacity; ยป State 3
- ยป OXPHOS-control ratio, P/E ratio
- ยป Respiratory acceptor control ratio
- ยป ROUTINE-control ratio
- ยป ROUTINE respiration
- ยป ROUTINE state
- ยป State 3u
- ยป State 4
- ยป Uncoupling-control ratio UCR
- Respiratory states and control ratios
- Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- General (alphabetical order)
- Other keyword lists
MitoPedia topics:
Uncoupler