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MitoEAGLE preprint 2017-09-21

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


MitoEAGLE preprint 2017-09-21


Publications in the MiPMap
MitoEAGLE preprint 2018-02-18(21) The protonmotive force and respiratory control.

ยป Bioblast pdf - ยป Versions

Published: ยป Gnaiger Erich et al โ€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1. - ยปBioblast linkยซ

MitoEAGLE (2018) MitoEAGLE

Abstract: Note: Subscript โ€˜ยงโ€™ indicates throughout the text those parts, where potential differences provide a mathematically correct but physicochemically incomplete description and should be replaced by stoichiometric potential differences (Gnaiger 1993b). A unified concept on vectorial motive transformations and scalar chemical reactions will be derived elsewhere (Gnaiger, in prep.). Appreciation of the fundamental distinction between differences of potential versus differences of stoichiometric potential may be considered a key to critically evaluate the arguments presented in Section 3 on the protonmotive force. Since this discussion appears to be presently beyond the scope of a MitoEAGLE position statement, Section 3 is removed from the next version and final manuscript. This section should become a topic of discussion within Working Group 1 of the MitoEAGLE consortium, following a primary peer-reviewed publication of the concept of stoichiometric potential differences. โ€ข Keywords: Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux


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ยป Manuscript phases and versions

Manuscript phases and versions - an open-access apporach

COST Action MitoEAGLE
This manuscript on โ€˜Mitochondrial respiratory states and ratesโ€™ is a position statement in the frame of COST Action CA15203 MitoEAGLE. The list of coauthors evolved beyond phase 1 in the bottom-up spirit of COST.
The global MitoEAGLE network made it possible to collaborate with a large number of coauthors to reach consensus on the present manuscript. Nevertheless, we do not consider scientific progress to be supported by โ€˜declarationโ€™ statements (other than on ethical or political issues). Our manuscript aims at providing arguments for further debate rather than pushing opinions. We hope to initiate a much broader process of discussion and want to raise the awareness on the importance of a consistent terminology for reporting of scientific data in the field of bioenergetics, mitochondrial physiology and pathology. Quality of research requires quality of communication. Some established researchers in the field may not want to re-consider the use of jargon which has become established despite deficiencies of accuracy and meaning. In the long run, superior standards will become accepted. We hope to contribute to this evolutionary process, with an emphasis on harmonization rather than standardization.
  • Phase 1: The protonmotive force and respiratory control
ยป The protonmotive force and respiratory control - Discussion
ยป MitoEAGLE preprint 2017-09-21 - Discussion
  • Phase 2: Mitochondrial respiratory states and rates: Building blocks of mitochondrial physiology Part 1
ยป MitoEAGLE Task Group States and rates - Discussion
  • Phase 4: Journal submission
  • Target: CELL METABOLISM, aiming at indexing by The Web of Science and PubMed.
Coauthors
  • 2017-09-21 Version 01: 105 coauthors
  • 2017-10-15 Version 10: 131 coauthors
  • 2018-01-18 Version 20: 168 coauthors
  • 2018-02-26 Version 30: 225 coauthors
  • 2018-08-20 Version 40: 350 coauthors - EBEC Poster
  • 2018-10-17 Version 44: 426 coauthors - MiPschool Tromso-Bergen 2018
  • 2018-12-12 Version 50: 517 coauthors - Submission to the preprint server bioRxiv not successful
  • 2019-02-12 Preprint version 1: 530 coauthors
  • 2019-03-15 Preprint version 2: 533 coauthors
  • 2019-04-24 Preprint version 3: 533 coauthors
  • 2019-05-20 Preprint version 4: 542 coauthors
  • 2019-07-24 Preprint version 5: 612 coauthors
  • 2019-08-30 Preprint version 6: 622 coauthors - Preprint publication doi:10.26124/mitofit:190001.v6
  • BEC 2020.1. - Gnaiger Erich et al โ€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1. - ยปBioblast linkยซ


Fig. 2. The proton circuit and coupling in oxidative phosphorylation (OXPHOS). Modified after Gnaiger 2014 MitoPathways.

Preprints for Gentle Science

Section 2: Oxidative phosphorylation and coupling states in mitochondrial preparations
Section 3: The protonmotive force and proton flux
Table Protonmotive force matrix.png
Fig. 8. Three formats of the protonmotive unit (A) and protonmotive force (B).
Table Physical constants.png

Table Power, exergy, force, flux, advancement.png


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Bioblast links: Force and membrane potential - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Fundamental relationships
ยป Force
ยป Affinity
ยป Flux
ยป Advancement
ยป Advancement per volume
ยป Stoichiometric number
mt-Membrane potential and protonmotive force
ยป Protonmotive force
ยป Mitochondrial membrane potential
ยป Chemical potential
ยป Faraday constant
ยป Format
ยป Uncoupler
O2k-Potentiometry
ยป O2k-Catalogue: O2k-TPP+ ISE-Module
ยป O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
ยป TPP - O2k-Procedures: Tetraphenylphosphonium
ยป Specifications: MiPNet15.08 TPP electrode
ยป Poster
ยป Unspecific binding of TPP+
ยป TPP+ inhibitory effect
O2k-Fluorometry
ยป O2k-Catalogue: O2k-FluoRespirometer
ยป O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
ยป Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
ยป TMRM - O2k-Procedures: TMRM
O2k-Publications
ยป O2k-Publications: mt-Membrane potential
ยป O2k-Publications: Coupling efficiency;uncoupling



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Bioblast links: pH and protons - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
pH and protons
ยป pH
ยป hydrogen ion H+
ยป hydron H+
ยป hydronium ion H3O+
ยป hydride H-
ยป proton p+
ยป pH buffering capacity
ยป proton flux
ยป proton pump versus hydrogen ion pump
ยป proton leak
ยป proton slip
ยป protonmotive force
O2k-pH
ยป O2k-Catalogue: O2k-pH ISE-Module
ยป O2k-Manual pH electrode: MiPNet23.15 O2k-pH ISE-Module
ยป O2k-SOP: MiPNet08.16 pH calibration
ยป File:PH-Calibration-List.xls
ยป NextGen-O2k, ratiometric: Carboxy SNARF 1
ยป NextGen-O2k, ratiometric: HPTS
ยป pH calibration buffers
O2k-Publications
ยป O2k-Publications: O2k-pH ISE-Module
HRFR - general
ยป O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
ยป O2k signals and output
ยป O2k-SOP: MiPNet14.06 Instrumental O2 background
ยป MiPNet19.18A O2k-Series G: Start
ยป ESD
ยป O2k configuration
ยป O2k control
ยป O2k-FluoRespirometer
ยป O2k-Main Unit#O2k-Series
ยป Titration-Injection microPump
ยป Compare: O2k-TPP+_ISE-Module
DatLab
ยป DatLab 7
ยป MiPNet26.06 DatLab 7: Guide
ยป DatLab 6
ยป MiPNet19.18C DatLab 6: Guide
ยป MiPNet19.18D O2k-Series G and DatLab 6: Calibration
ยป Reference layouts for DatLab graphs



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MitoEAGLEPublication