Mitochondrial membrane potential: Difference between revisions
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== Different methods for measurements of mt-membrane potential == | == Different methods for measurements of mt-membrane potential == | ||
:::: mt-Membrane potential can either be measured in the [[O2k-FluoRespirometer]] fluorometrically by using the fluorophores [[TMRM]] | :::: mt-Membrane potential can either be measured in the [[O2k-FluoRespirometer]] fluorometrically by using the fluorophores [[TMRM]], [[Safranin]], or [[Rhodamine 123]] or potentiometrically with the [[O2k-TPP+ ISE-Module]] electrode by using the ion reporter [[TPP+]]. All mentioned ion indicator molecules inhibit respiration, which makes it essential to test the optimum concentration. | ||
== High-resolution respirometry and mt-membrane potential == | == High-resolution respirometry and mt-membrane potential == |
Revision as of 09:05, 24 September 2020
- high-resolution terminology - matching measurements at high-resolution
Mitochondrial membrane potential
Description
The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ΞpH+.
ΞΟ = ΞpH+ - ΞΒ΅H+ / F
mtMP or ΞΟ is the potential difference across the inner mitochondrial (mt) membrane, expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per βmotiveβ electron or per electron moved across the transmembrane potential difference, with the number of βmotiveβ electrons expressed in the unit coulomb [C].
Abbreviation: mtMP, ΞΟ [V]
Reference: Mitchell 1961 Nature, Gnaiger 2014 Preface MiP2014
Communicated by Gnaiger E 2012-10-05, edited 2016-02-06, 2017-09-05.
- The chemical part of the protonmotive force, Β΅H+ / F stems from the difference of pH across the mt-membrane. It contains a factor that bridges the gap between the electric force [J/C] and the chemical force [J/mol]. This factor is the Faraday constant, F, for conversion between electric force expressed in joules per coulomb or Volt [V=J/C] and chemical force with the unit joules per mole or Jol [Jol=J/mol],
- F = 96.4853 kJol/V = 96,485.3 C/mol
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Different methods for measurements of mt-membrane potential
- mt-Membrane potential can either be measured in the O2k-FluoRespirometer fluorometrically by using the fluorophores TMRM, Safranin, or Rhodamine 123 or potentiometrically with the O2k-TPP+ ISE-Module electrode by using the ion reporter TPP+. All mentioned ion indicator molecules inhibit respiration, which makes it essential to test the optimum concentration.
High-resolution respirometry and mt-membrane potential
- The O2k-MultiSensor system provides a potentiometric and a fluorometric module for measurement of the mt-membrane potential.
- Β» O2k-Manual TPP: MiPNet15.03 O2k-MultiSensor-ISE
- Β» TPP: O2k-technical_support
- Β» MiPNet20.13 Safranin mt-membranepotential
- Β» TMRM: O2k-technical_support
Calculations
- Data analysis of mitochondrial membrane potential estimation using various fluorescence dyes: MiPNet24.09 General Template for Mt-membrane Potential Analysis to calculate the relative mt-membrane potential values.
- Data analysis of mitochondrial membrane potential estimation with safranin using DatLab 7.4: MiPNet24.08 Safranin Analysis Template to express the mt-membrane potential values in mV.
- The calculation used to calculate the mt-membrane potential values are provided here complying with Oroboros transparancy policy, see the following page: Safranin
- for detailed explanaition, see MiPNet24.11 mtMP calculation
SUITbrowser question: Mitochondrial membrane potential
- Several SUIT protocols are focused on the measurement of mt-membrane potential by potentiometric and fluorometric techniques.
- Use the SUITbrowser to find the best protocol to answer this and other research questions.
Keywords: Force and membrane potential
- Bioblast links: Force and membrane potential - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Fundamental relationships
- Β» Force
- Β» Affinity
- Β» Flux
- Β» Advancement
- Β» Advancement per volume
- Β» Stoichiometric number
- Fundamental relationships
- mt-Membrane potential and protonmotive force
- Β» Uncoupler
- O2k-Potentiometry
- Β» O2k-Catalogue: O2k-TPP+ ISE-Module
- Β» O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
- Β» TPP - O2k-Procedures: Tetraphenylphosphonium
- Β» Specifications: MiPNet15.08 TPP electrode
- Β» Poster
- Β» Unspecific binding of TPP+
- Β» TPP+ inhibitory effect
- O2k-Potentiometry
- O2k-Fluorometry
- Β» O2k-Catalogue: O2k-FluoRespirometer
- Β» O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
- Β» Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
- Β» TMRM - O2k-Procedures: TMRM
- O2k-Fluorometry
- O2k-Publications
MitoPedia concepts:
Respiratory state,
Recommended,
Ergodynamics
MitoPedia methods:
Respirometry,
Fluorometry
MitoPedia topics:
EAGLE
Labels:
MitoPedia:NextGen-O2k