MitoPedia: SUIT A

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MitoPedia: SUIT A

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MitoPedia: SUIT is a component of OROBOROS support.

In the Library of SUIT protocols

» MitoPedia: SUIT A - these are SUIT protocols used for cohort studies.

» MitoPedia: SUIT B - these are published SUIT protocols considered less suitable for cohort studies.

» MitoPedia: SUIT C - these are SUIT protocols used for exploratory research.

» MitoPedia: SUIT - you are here: this is a list of all SUIT protocols in the library of SUIT protocols.

See also:

» O2k-Protocols: O2k-Demo experiments

Library of SUIT protocols: all

Short name	Systematic name	Coupling/pathway control diagram	Reference
1OctM;2D;3PG;4S;5U;6Rot-	FNS(Oct,PGM)		C MiPNet18.13 IOC84 Alaska
1PGM;2D;3S;4Rot;5U-	NS(PGM)		C
1PGM;2D;3U;4S;5Rot-	NS(PGM)		C MiPNet18.13 IOC84 Alaska
1PM;2D;3G;4U;5S;6Rot-	NS(PGM)		B Lemieux 2011 Int J Biochem Cell Biol
SUIT-001	RP1		Doerrier 2018 Methods Mol Biol
SUIT-001 O2 ce-pce D003	RP1 ce-pce		A - SUIT-001 - SUIT reference protocol Reference protocol RP1 for living cells (ce, ROUTINE respiration) and cells permeabilized in the respirometric chamber (pce)
SUIT-001 O2 ce-pce D004	RP1 ce-pce blood		A - SUIT-001 - SUIT reference protocol Reference protocol RP1 for living PBMC and PLT cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)
SUIT-001 O2 mt D001	RP1 mt		SUIT-001 - SUIT reference protocol Reference protocol RP1 for isolated mitochondria, tissue homogenate and permeabilized cells
SUIT-001 O2 pfi D002	RP1 pfi		SUIT-001 - SUIT reference protocol Reference protocol RP1 for permeabilized fibers
SUIT-002	RP2		A: Doerrier 2018 Methods Mol Biol
SUIT-002 O2 ce-pce D007	RP2 ce-pce		A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for living cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)
SUIT-002 O2 ce-pce D007a	RP2 ce-pce blood		A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for for living PBMC and PLT cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)
SUIT-002 O2 mt D005	RP2 mt		A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for isolated mitochondria, tissue homogenate and permeabilized cells
SUIT-002 O2 pfi D006	RP2 pfi		A -SUIT-002 - SUIT reference protocol Reference protocol RP2 for permeabilized fibers (pfi)
SUIT-003	CCP-ce		A: Coupling-control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003 AmR ce D017	CCP-ce S permeability test		B: CCP(P) and cell membrane permeability test with succinate - SUIT-003
SUIT-003 AmR ce D058	AmR effect on ce		B: Coupling control in intact cells with oligomycin to study the inhibitory effect of the AmR assay components on the respiration ; ce: non-permeabilized cells -SUIT-003
SUIT-003 AmR ce D059	AmR effect on ce - control		B: Experimental control for SUIT-003 AmR ce D058
SUIT-003 Ce1;ce1P;ce3U;ce4Glc;ce5M;ce6Rot;ce7S;1Dig;1c;2Ama;3AsTm;4Azd	cePMGlc,S		C SUIT-003 Coupling-control protocol and respirometric cell viability test (CCV protocol) without oligomycin.
SUIT-003 Ce1;ce1SD;ce2Omy;ce3U-	FNS(Oct,PGM)		B SUIT-003 Stadlmann 2006 Cell Biochem Biophys
SUIT-003 Ce1;ce1SD;ce3U;ce4Rot;ce5Ama	ceS		B SUIT-003 CCP(S)
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-	FNS(Oct,PGM)		B SUIT-003 Stadlmann 2006 Cell Biochem Biophys
SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama	ceS		B Steinlechner-Maran 1997 Transplantation
SUIT-003 Ce1;ce2U;ce3Rot;ce4S;ce5Ama	ceS		B SUIT-003 CCP(S)
SUIT-003 Ce1;ce3U;ce4Rot;ce5S;ce6Ama	ceS		B SUIT-003 CCP(S)
SUIT-003 O2 ce D009	CCP-ce short		A: - SUIT-003 - Coupling-control protocol (CCP) in living cells (ce) with oligomycin (LEAK state)
SUIT-003 O2 ce D012	CCP-ce(P)		B - SUIT-003
SUIT-003 O2 ce D028	CCP-ce S permeability test		B SUIT-003 Coupling-control protocol with succinate for permeability test
SUIT-003 O2 ce D037	CCP-ce Crabtree_R		A SUIT-003 - Coupling-control protocol and Crabtree effect
SUIT-003 O2 ce D038	CCP-ce Crabtree_E		A Coupling-control protocol and Crabtree effect test -SUIT-003 complex cell coupling-control protocol
SUIT-003 O2 ce D039	CCP-ce microalgae		A: Coupling control in intact cells without oligomycin; ce: non-permeabilized cells -SUIT-003
SUIT-003 O2 ce D050	CCP-ce Snv		A: - Coupling-control protocol for living cells with plasma membrane-permeable succinate (MitoKit-CII/Succinate-nv) - SUIT-003 - Plasma membrane-permeable succinate (NV118) in ROUTINE respiration. Coupling-control protocol (CCP) in living cells (ce)
SUIT-003 O2 ce D060	CCP-ce Snv,Mnanv		B - coupling-control protocol for living cells with cell permeable compounds MitoKit-CII/Succinate-nv and MitoKit-CII/Malonate-nv -SUIT-003 - Coupling-control protocol (CCP) with plasma membrane-permeable succinate (MitoKit-CII/Succinate-nv) and plasma membrane-permeable malonate (MitoKit-CII/Malonate-nv) in living cells (ce).
SUIT-003 O2 ce D061	CCP-ce Snv,Mnanv - control		B Carrier control for the coupling-control protocol for living cells with cell permeable compounds MitoKit-CII/Succinate-nv and MitoKit-CII/Malonate-nv - SUIT-003 O2 ce D060
SUIT-003 O2 ce D062	CCP-ce Snv - control		A: Carrier control for the Coupling-control protocol for living cells with plasma membrane-permeable succinate (MitoKit-CII/Succinate-nv) - SUIT-003 O2 ce D050
SUIT-003 O2 ce-pce D013	CCVP-Glc,M		C - SUIT-003
SUIT-003 O2 ce-pce D018	CCVP-Glc		C SUIT-003 Second version of the Coupling-control protocol and respirometric cell viability test (CCV protocol)
SUIT-003 O2 ce-pce D020	CCVP		A SUIT-003 - Coupling-control protocol and respirometric cell viability test; ce: living cells, pce: permeabilized cells
SUIT-003 pH ce D067	CCP-Crabtree with glycolysis inhibition		A Coupling-control protocol and Crabtree effect with glycolysis inhibition- SUIT-003
SUIT-004	RP1-short
SUIT-004 O2 pfi D010	RP1-short pfi		SUIT-004 - Short protocol linked to SUIT-001 O2 pfi D002 SUIT RP1 (human skeletal muscle)
SUIT-005	RP2-short		A: when malate-anaplerotic activity is zero
SUIT-005 O2 pfi D011	RP2-short pfi		A: - SUIT-005 short protocol linked to SUIT-002 O2 pfi D006 - SUIT RP2 (human skeletal muscle)
SUIT-006	CCP-mtprep		A: Coupling-control protocol, mtprep
SUIT-006 AmR mt D048	CCP mt PM - AmR		A: short protocol for simultaneous determination of O2 flux and H2O2 in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- SUIT-006
SUIT-006 Fluo mt D034	CCP mt PM - Fluo		A: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006
SUIT-006 MgG ce-pce D085	CCP MgG ce-pce		A: short coupling-control protocol for simultaneous determination of O2 flux and mitochondrial ATP production in permeabilized cells -SUIT-006
SUIT-006 MgG mt D055	CCP MgG mt		A: short coupling-control protocol for simultaneous determination of O2 flux and mitochondrial ATP production in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006
SUIT-006 O2 ce-pce D029	CCP ce-pce PM		A to analyse the coupling control on permeabilized cells in N(PM) pathway control state- SUIT-006
SUIT-006 O2 mt D022	CCP mt S(Rot)		A - SUIT-006 - Protocol for coupling control assessment in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)
SUIT-006 O2 mt D047	CCP mt PM		A SUIT-006 - is a coupling-control protocol in the N-pathway for isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k chamber.
SUIT-006 Q ce-pce D073	CCP ce-pce S(Rot)		A Coupling control protocol to analyze mitochondrial O2 consumption and the redox state of the Q-pool in S(Rot)-pathway control state in permeabilized cells- SUIT-006
SUIT-006 Q mt D071	CCP mt S(Rot)		A Coupling-control protocol to analyze mitochondrial O2 consumption and the redox state of the Q-pool in S(Rot)-pathway control state in isolated mitochondria- SUIT-006
SUIT-007	Glutamate anaplerosis		A: Glutamate anaplerotic pathway
SUIT-007 O2 ce-pce D030	Glutamate anaplerotic pathway		A pce: permeabilized cells - SUIT-007
SUIT-008	PM+G+S_OXPHOS+Rot_ET		A: Additivity between the N- and S-pathway in the Q-junction
SUIT-008 O2 ce-pce D025	Q-junction ce-pce		A: for cells-permeabilized cells Lemieux 2017 Sci Rep - SUIT-008
SUIT-008 O2 mt D026	Q-junction mtprep		A: SUIT protocol for mt; mt: isolated mitochondria and tissue homogenate - SUIT-008
SUIT-008 O2 pce D25	NS(PGM)		B: SUIT 8 for permeabilized cells Lemieux 2017 Sci Rep
SUIT-008 O2 pfi D014			A - Lemieux 2017 Sci Rep - SUIT-008- O2 pfi D014 protocol was used a a reference protocol in MitoEAGLE project (WG2) to evaluate the researcher skills preparing permeabilized fibers
SUIT-009 AmR ce-pce D019	H2O2 RET ce-pce S_L		B: short protocol for H2O2 (AmR) in ce: non-permeabilized cells, pce: permeabilized cells -SUIT-009
SUIT-009 AmR mt D021	H2O2 mtprep		B: short protocol for H2O2 (AmR) in mitochondrial preparations; mt: isolated mitochondria, tissue homogenate and permeabilized cells - SUIT-009short protocol for H2O2 (AmR) for isolated mitochondria, tissue homogenate and permeabilized cells
SUIT-009 O2 ce-pce D016			B - SUIT-009 - short protocol for mt-respiration in ce: non-permeabilized cells, pce: permeabilized cells.
SUIT-009 O2 mt D015			B - SUIT-009- short protocol for respiration in mitochondrial preparations; mt: isolated mitochondria, tissue homogenate and permeabilized cells
SUIT-010	Digitonin test		A: Optimization of digitonin concentration for pce
SUIT-010 O2 ce-pce D008	Dig titration-pce		A -SUIT-010 - Optimum digitonin concentration for pce
SUIT-011	GM+S_OXPHOS+Rot_ET		A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control
SUIT-011 O2 pfi D024	NS physiological maximum capapcity in fibres		A pfi: permeabilized fibers - SUIT-011
SUIT-012	PM+G_OXPHOS		A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
SUIT-012 O2 ce-pce D052	N(PGM)		A Linear coupling control with NADH-linked substrates for pce - SUIT-012
SUIT-012 O2 mt D027	N CCP mtprep		A Linear coupling control with NADH-linked substrates for isolated mitochondria, tissue homogenate and permeabilized cells- SUIT-012
SUIT-013	ce		A
SUIT-013 AmR ce D023	O2 dependence of H2O2 production ce		A SUIT-013
SUIT-014	GM+P+S_OXPHOS+Rot_ET		A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
SUIT-014 O2 pfi D042	NS(PGM)		A SUIT-014
SUIT-015	F+G+P+S_OXPHOS+Rot_ET		A: F-pathway in LEAK state and OXPHOS state
SUIT-015 O2 pti D043	FNS(Oct,PGM)		A pti: permeabilized tissue- SUIT-015
SUIT-016	F+G+S+Rot_OXPHOS+Omy		A: F-pathway in LEAK state and OXPHOS state
SUIT-016 O2 pfi D044	FNS(Oct,GM)		A pfi: permeabilized fibers- SUIT-016
SUIT-017	F+G+S_OXPHOS+Rot_ET		A:
SUIT-017 O2 mt D046	FNS(Oct,GM)		A: SUIT-017
SUIT-017 O2 pfi D049	FNS(Oct,GM)		A: SUIT-017
SUIT-018	O2 dependence-H2O2		A: A protocol for oxygen dependence of O2 flux and H2O2 flux in isolated mitochondria,tissue homogenate or permeabilized cells
SUIT-018 AmR ce-pce D068	O2 dependence-H2O2		A -SUIT-018 - SUIT protocol for simultaneous determination of O2 and H2O2 flux in permeabilized cells at various oxygen concentrations
SUIT-018 AmR mt D031	O2 dependence-H2O2		A -SUIT-018 - SUIT protocol for simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) at low oxygen concentration (tissue normoxia)
SUIT-018 AmR mt D040	NS(GM)		A: simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)-SUIT-018
SUIT-018 AmR mt D041	O2 dependence-H2O2		A -SUIT-018
SUIT-018 O2 mt D054			B: SUIT-018
SUIT-019	Pal+Oct+P+G_OXPHOS+S+Rot_ET		A:
SUIT-019 O2 pfi D045	FNS(PalOct,PGM)		A SUIT-019
SUIT-020	PM+G+S+Rot_OXPHOS+Omy		A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-020 Fluo mt D033	NS(PGM)		A:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)- SUIT-020
SUIT-020 O2 mt D032	Q-junction additivity and respiratory control for membrane potential		A: short protocol for determination of O2 flux in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-020
SUIT-021	OXPHOS (GM+S+Rot+Omy)		A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-021 Fluo mt D036	NS(GM)		A: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-021
SUIT-021 O2 mt D035	NS(GM)		A:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permabilized cells, and tissue homogenate)-SUIT-021
SUIT-022	AOX (ce CN+SHAM)		A: Determination of the respiration due to the alternative oxidase pathway
SUIT-022 O2 ce D051	AOX-ce CN+SHAM		A Determination of the respiration due to the alternative oxidase pathway -SUIT-022
SUIT-023	AOX-ce SHAM+CN		A: Determination of respiration through the CIII-CIV pathway
SUIT-023 O2 ce D053	AOX-ce SHAM+CN		A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023
SUIT-024	ATPase (PM)		A: Determination of ATPase activity in mitochondrial preparations.
SUIT-024 O2 ce-pce D056	N(PM)		Determination of the presence of ATPases in mitochondrial preparations - SUIT-024
SUIT-025	OXPHOS (F+M+P+G+S+Rot)
SUIT-025 O2 mt D057	FNS(Oct,PGM)		- SUIT-025
SUIT-026	RET		A Short protocol to study RET-related H2O2 production
SUIT-026 AmR ce-pce D087	RET		A - SUIT-026 - Protocol for the evaluation of ROS production by RET in permeabilized cells
SUIT-026 AmR mt D064	RET		A - SUIT-026 - Protocol for the evaluation of ROS production by RET in mtprep
SUIT-026 AmR mt D077	RET		A - SUIT-026 - Protocol for the evaluation of ROS production by RET in mtprep
SUIT-026 O2 ce-pce D088	RET (respiratory control) of SUIT-026 AmR ce-pce D087	400px	B - SUIT-026 - Protocol for the respiratory control of SUIT-026 AmR ce-pce D087
SUIT-026 O2 mt D063	RET (respiratory control) of SUIT-026 AmR mt D064		B - SUIT-026 - Protocol for the respiratory control of SUIT-026 AmR mt D064
SUIT-027	Malate anaplerosis		A: Malate anaplerotic pathway
SUIT-027 O2 ce-pce D065	Malate anaplerosis		A - SUIT-027 - Protocol for analysis of the malate anaplerotic pathway
SUIT-028	NS(PGM)		C MiPNet18.13 IOC84 Alaska
SUIT-029 O2 mt D066	QC_imt_PM_T+OXPHOS+c+Omy_ET_G+S+Rot		A: quality control evaluation of mitochondrial preparations - SUIT-029
SUIT-031	PM+S+Rot		A:
SUIT-031 O2 ce-pce D079	PM+S+Rot		A to analyse O2 flux in the NS- pathway control state in permeabilized cells- SUIT-031
SUIT-031 O2 mt D075	PM+S+Rot		A to analyse O2 flux in the NS- pathway control state on isolated mitochondria- SUIT-031
SUIT-031 Q ce-pce D074	PM+S+Rot		A to analyse O2 flux and the Q-redox state in the NS- pathway control state on permeabilized cells- SUIT-031
SUIT-031 Q mt D072	PM+S+Rot		A to analyse O2 flux and the Q-redox state in the N-,NS- and S- pathway control state on isolated mitochondria- SUIT-031
SUITbrowser		Use the SUITbrowser to find the substrate-uncoupler-inhibitor-titration (SUIT) protocol most suitable for addressing your research questions. Open the SUITbrowser: http://suitbrowser.oroboros.at/ How to find a DL-Protocol (DLP)