Difference between revisions of "Mitochondrial membrane potential"
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{{MitoPedia | {{MitoPedia | ||
|abbr=mtMP, Î'' | |abbr=mtMP, Î''Κ''<sub>p<sup>+</sup></sub>, Î<sub>el</sub>''F''<sub><u>''e''</u>p<sup>+</sup></sub> [V] | ||
|description=The '''mitochondrial membrane potential''', mtMP, is the electric part of the protonmotive [[force]], Î'' | |description=The '''mitochondrial membrane potential''' difference, mtMP or Î''Κ''<sub>p<sup>+</sup></sub> = Î<sub>el</sub>''F''<sub><u>''e''</u>p<sup>+</sup></sub>, is the electric part of the protonmotive [[force]], Îp = Î<sub>m</sub>''F''<sub><u>''e''</u>H<sup>+</sup></sub>. | ||
Î'' | :::: Î<sub>el</sub>''F''<sub><u>''e''</u>p<sup>+</sup></sub> = Î<sub>m</sub>''F''<sub><u>''e''</u>H<sup>+</sup></sub> - Î<sub>d</sub>''F''<sub><u>''e''</u>H<sup>+</sup></sub> | ||
:::: Î''Κ''<sub>p<sup>+</sup></sub> = Îp - Î''”''<sub>H+</sub>·(''z''<sub>H<sup>+</sup></sub>·''F'')<sup>-1</sup> | |||
Î''Κ''<sub>p<sup>+</sup></sub> is the potential difference across the mitochondrial inner membrane (mtIM), expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per âmotiveâ charge or per charge moved across the transmembrane potential difference, with the number of âmotiveâ charges expressed in the unit coulomb [C]. | |||
|info=[[Mitchell 1961 Nature]], [[Gnaiger 2020 BEC MitoPathways]] | |||
}} | |||
Communicated by [[Gnaiger E]] 2012-10-05, edited 2016-02-06, 2017-09-05, 2022-07-05. | |||
:::: The chemical part of the protonmotive force, Î<sub>d</sub>''F''<sub><u>''e''</u>H<sup>+</sup></sub> = Î''”''<sub>H+</sub>·(''z''<sub>H<sup>+</sup></sub>·''F'')<sup>-1</sup>, stems from the difference of pH across the mt-membrane. It contains a factor that bridges the gap between the electric force [J/C] and the chemical force [J/mol]. This factor is the Faraday constant, ''F'', for conversion between electric force expressed in joules per coulomb or Volt [V=J/C] and chemical force with the unit joules per mole or Jol [Jol=J/mol], | |||
:::::::: ''F'' = 96.4853 kJol/V = 96,485.3 C/mol | |||
__TOC__ | |||
{{Technical support integrated}} | |||
{{ | |||
== Different methods for measurements of mt-membrane potential == | == Different methods for measurements of mt-membrane potential == | ||
:::: | :::: Mt-membrane potential can either be measured with the [[O2k-Fluo Smart-Module]] fluorometrically by using the fluorophores [[TMRM]], [[Safranin]], or [[Rhodamine 123]] or potentiometrically with the [[O2k-TPP+ ISE-Module]] electrode by using the ion reporter [[TPP+]]. All mentioned ion indicator molecules inhibit and may uncouple respiration, which makes it essential to test the optimum concentration. | ||
== High-resolution respirometry and mt-membrane potential == | == High-resolution respirometry and mt-membrane potential == | ||
:::: The O2k-MultiSensor system provides a potentiometric and a fluorometric module for measurement of the mt-membrane potential. | :::: The O2k-MultiSensor system provides a potentiometric and a fluorometric module for measurement of the mt-membrane potential. | ||
::::» O2k-Manual TPP: [[MiPNet15.03 O2k-MultiSensor-ISE]] | |||
::::» [[Tetraphenylphosphonium#O2k-technical_support |TPP: O2k-technical_support]] | |||
::::» [[MiPNet20.13 Safranin mt-membranepotential |MiPNet20.13 Safranin mt-membrane potential]] | |||
::::» [[TMRM#O2k-technical_support |TMRM: O2k-technical_support]] | |||
== Calculations == | |||
::::* Data analysis of [[Mitochondrial membrane potential|mitochondrial membrane potential]]Â estimation using various fluorescence dyes: [[MiPNet24.09_Data_analysis_of_mt-membrane_potential|MiPNet24.09 Data analysis of mt-membrane potential]] to calculate the relative mt-membrane potential values. | |||
::::* Data analysis of [[Mitochondrial membrane potential|mitochondrial membrane potential]] estimation with safranin using DatLab 7.4: [[MiPNet24.08 Safranin Analysis Template]] to express the mt-membrane potential values in mV. | |||
:::: | |||
:::: | :::::* The equations used to estimate the mt-membrane potential values are provided here complying with Oroboros transparency policy, see the following page: [[Safranin#Analysis_of_mitochondrial_membrane_potential_using_safranin|Safranin]] | ||
=== | :::::* for detailed explanation, see [[MiPNet24.11 mtMP calculation]] | ||
::::The | == Excel analysis templates == | ||
:::* More advanced Excel analysis templates for the respective SUIT protocols to calculate the relative mt-membrane potential values are available on this page. | |||
::::* The calculations used in the excel analysis template are provided complying with Oroboros transparency policy: [https://wiki.oroboros.at/index.php/Flux_/_Slope] | |||
:::: | ::::*''Last update 2021-09-06'': Chemical background correction was implemented into the Excel analysis templates. | ||
:::: | ::::* For [[SUIT-006 Fluo mt D034]] protocol, see template: [[File:SUIT-006 Fluo mt D034 general.xlsx]] and a demo [[File:SUIT-006 Fluo mt D034 general demo.xlsx]] | ||
:: | ::::* For [[SUIT-020 Fluo mt D033]] protocol, see template: [[File:SUIT-020 Fluo mt D033 general.xlsx]] and a demo [[File:SUIT-020 Fluo mt D033 general demo.xlsx]] | ||
:::: | ::::* For [[SUIT-021 Fluo mt D036]] protocol, see template: [[File:SUIT-021 Fluo mt D036 general.xlsx]] and a demo [[File:SUIT-021 Fluo mt D036 general demo.xlsx]] | ||
:::: | |||
== | :::* Manual: [[MiPNet24.09 General Template for Mt-membrane Potential Analysis|MiPNet24.09 Data analysis of mt-membrane potential]] | ||
:::: | == [[SUITbrowser]] question: Mitochondrial membrane potential == | ||
:::: | :::: Several [[SUIT]] protocols are focused on the measurement of mt-membrane potential by potentiometric and fluorometric techniques. | ||
:::: Use the [https://suitbrowser.oroboros.at/ SUITbrowser] to find the best protocol to answer this and other research questions. | |||
== | == O2k-Publications: mtMP == | ||
::: | [[Image:O2k-Publications.jpg|left|150px|link=http://www.bioblast.at/index.php/O2k-Publications: Â Topics|O2k-Publications in the MiPMap]] | ||
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''. | |||
Default sorting: chronological. Empty fields appear first in ascending order. | |||
{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::mt-Membrane potential]] | |||
|?Was published in year=Year | |||
|?Has title=Reference | |||
|?Mammal and model=Organism | |||
|?Tissue and cell=Tissue;cell | |||
|format=broadtable | |||
|limit=5000 | |||
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|sort=Was published in year | |||
|order=descending | |||
}} | |||
== | == Keywords: Force and membrane potential == | ||
 | {{Keywords: Force and membrane potential}} | ||
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|mitopedia concept=Respiratory state, Recommended, Ergodynamics | |mitopedia concept=Respiratory state, Recommended, Ergodynamics | ||
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|mitopedia topic=EAGLE | |mitopedia topic=EAGLE | ||
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|additional=MitoPedia:NextGen-O2k | |||
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Latest revision as of 17:02, 17 January 2024
- high-resolution terminology - matching measurements at high-resolution
Mitochondrial membrane potential
Description
The mitochondrial membrane potential difference, mtMP or ÎΚp+ = ÎelFep+, is the electric part of the protonmotive force, Îp = ÎmFeH+.
- ÎelFep+ = ÎmFeH+ - ÎdFeH+
- ÎΚp+ = Îp - ΔH+·(zH+·F)-1
ÎΚp+ is the potential difference across the mitochondrial inner membrane (mtIM), expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per âmotiveâ charge or per charge moved across the transmembrane potential difference, with the number of âmotiveâ charges expressed in the unit coulomb [C].
Abbreviation: mtMP, ÎΚp+, ÎelFep+ [V]
Reference: Mitchell 1961 Nature, Gnaiger 2020 BEC MitoPathways
Communicated by Gnaiger E 2012-10-05, edited 2016-02-06, 2017-09-05, 2022-07-05.
- The chemical part of the protonmotive force, ÎdFeH+ = ΔH+·(zH+·F)-1, stems from the difference of pH across the mt-membrane. It contains a factor that bridges the gap between the electric force [J/C] and the chemical force [J/mol]. This factor is the Faraday constant, F, for conversion between electric force expressed in joules per coulomb or Volt [V=J/C] and chemical force with the unit joules per mole or Jol [Jol=J/mol],
- F = 96.4853 kJol/V = 96,485.3 C/mol
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Different methods for measurements of mt-membrane potential
- Mt-membrane potential can either be measured with the O2k-Fluo Smart-Module fluorometrically by using the fluorophores TMRM, Safranin, or Rhodamine 123 or potentiometrically with the O2k-TPP+ ISE-Module electrode by using the ion reporter TPP+. All mentioned ion indicator molecules inhibit and may uncouple respiration, which makes it essential to test the optimum concentration.
High-resolution respirometry and mt-membrane potential
- The O2k-MultiSensor system provides a potentiometric and a fluorometric module for measurement of the mt-membrane potential.
- » O2k-Manual TPP: MiPNet15.03 O2k-MultiSensor-ISE
- » TPP: O2k-technical_support
- » MiPNet20.13 Safranin mt-membrane potential
- » TMRM: O2k-technical_support
Calculations
- Data analysis of mitochondrial membrane potential estimation using various fluorescence dyes: MiPNet24.09 Data analysis of mt-membrane potential to calculate the relative mt-membrane potential values.
- Data analysis of mitochondrial membrane potential estimation with safranin using DatLab 7.4: MiPNet24.08 Safranin Analysis Template to express the mt-membrane potential values in mV.
- The equations used to estimate the mt-membrane potential values are provided here complying with Oroboros transparency policy, see the following page: Safranin
- for detailed explanation, see MiPNet24.11 mtMP calculation
Excel analysis templates
- More advanced Excel analysis templates for the respective SUIT protocols to calculate the relative mt-membrane potential values are available on this page.
- The calculations used in the excel analysis template are provided complying with Oroboros transparency policy: [1]
- Last update 2021-09-06: Chemical background correction was implemented into the Excel analysis templates.
- For SUIT-006 Fluo mt D034 protocol, see template: File:SUIT-006 Fluo mt D034 general.xlsx and a demo File:SUIT-006 Fluo mt D034 general demo.xlsx
- For SUIT-020 Fluo mt D033 protocol, see template: File:SUIT-020 Fluo mt D033 general.xlsx and a demo File:SUIT-020 Fluo mt D033 general demo.xlsx
- For SUIT-021 Fluo mt D036 protocol, see template: File:SUIT-021 Fluo mt D036 general.xlsx and a demo File:SUIT-021 Fluo mt D036 general demo.xlsx
SUITbrowser question: Mitochondrial membrane potential
- Several SUIT protocols are focused on the measurement of mt-membrane potential by potentiometric and fluorometric techniques.
- Use the SUITbrowser to find the best protocol to answer this and other research questions.
O2k-Publications: mtMP
Sort in ascending/descending order by a click on one of the small symbols in squares below. Default sorting: chronological. Empty fields appear first in ascending order.
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Ravasz 2024 Sci Rep | 2024 | Ravasz D, Bui D, Nazarian S, Pallag G, Karnok N, Roberts J, Marzullo BP, Tennant DA, Greenwood B, Kitayev A, Hill C, KomlĂłdi T, Doerrier C, Cunatova K, Fernandez-Vizarra E, Gnaiger E, Kiebish Michael A, Raska A, Kolev K, Czumbel B, Narain NR, Seyfried TN, Chinopoulos C (2024) Residual Complex I activity and amphidirectional Complex II operation support glutamate catabolism through mtSLP in anoxia. Sci Rep 14:1729. https://doi.org/10.1038/s41598-024-51365-4 | Mouse | Heart Liver |
Donnelly 2024 Redox Biol | 2024 | Donnelly C, KomlĂłdi T, Cecatto C, Cardoso LHD, Compagnion A-C, Matera A, Tavernari D, Campiche O, Paolicelli RC, Zanou N, Kayser B, Gnaiger E, Place N (2024) Functional hypoxia reduces mitochondrial calcium uptake. Redox Biol 71:103037. https://doi.org/10.1016/j.redox.2024.103037 | Human Mouse | Heart Skeletal muscle |
Krause 2023 J Transl Med | 2023 | Krause J, Nickel A, Madsen A, Aitken-Buck HM, Stoter AMS, Schrapers J, Ojeda F, Geiger K, Kern M, Kohlhaas M, Bertero E, Hofmockel P, HĂŒbner F, Assum I, Heinig M, MĂŒller C, Hansen A, Krause T, Park DD, Just S, AĂŻssi D, Börnigen D, Lindner D, Friedrich N, Alhussini K, Bening C, Schnabel RB, Karakas M, Iacoviello L, Salomaa V, Linneberg A, Tunstall-Pedoe H, Kuulasmaa K, Kirchhof P, Blankenberg S, Christ T, Eschenhagen T, Lamberts RR, Maack C, Stenzig J, Zeller T (2023) An arrhythmogenic metabolite in atrial fibrillation. https://doi.org/10.1186/s12967-023-04420-z | Human | Heart |
Donnelly 2023 MitoFit | 2023 | Donnelly C, KomlĂłdi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 â 2024-11-17 published in Redox Biol. | Human Mouse | Skeletal muscle Heart Nervous system Other cell lines |
Dabrowska 2023 Int J Mol Sci | 2023 | Dabrowska A, Zajac M, Bednarczyk P, Lukasiak A (2023) Effect of quercetin on mitoBKCa channel and mitochondrial function in human bronchial epithelial cells exposed to particulate matter. Int J Mol Sci 24:638. https://doi.org/10.3390/ijms24010638 | Human | Lung;gill Endothelial;epithelial;mesothelial cell |
Som 2023 Am J Physiol Cell Physiol | 2023 | Som R, Fink BD, Yu L, Sivitz WI (2023) Oxaloacetate regulates complex II respiration in brown fat: dependence on UCP1 expression. Am J Physiol Cell Physiol 324:C1236-48. doi: 10.1152/ajpcell.00565.2022 | Mouse | Fat |
Pallag 2022 Int J Mol Sci | 2022 | Pallag G, Nazarian S, Ravasz D, Bui D, KomlĂłdi T, Doerrier C, Gnaiger E, Seyfried TN, Chinopoulos C (2022) Proline oxidation supports mitochondrial ATP production when Complex I is inhibited. https://doi.org/10.3390/ijms23095111 | Mouse | Liver Kidney |
Tomar 2022 Biochim Biophys Acta Bioenerg | 2022 | Tomar N, Zhang X, Kandel SM, Sadri S, Yang C, Liang M, Audi SH, Cowley AW Jr, Dash RK (2022) Substrate-dependent differential regulation of mitochondrial bioenergetics in the heart and kidney cortex and outer medulla. https://doi.org/10.1016/j.bbabio.2021.148518 | Rat | Heart Kidney |
Ceja-Galicia 2022 Antioxidants (Basel) | 2022 | Ceja-Galicia ZA, GarcĂa-Arroyo FE, Aparicio-Trejo OE, El-Hafidi M, Gonzaga-SĂĄnchez G, LeĂłn-Contreras JC, HernĂĄndez-Pando R, Guevara-Cruz M, Tovar AR, Rojas-Morales P, Aranda-Rivera AK, SĂĄnchez-Lozada LG, Tapia E, Pedraza-Chaverri J (2022) Therapeutic effect of curcumin on 5/6Nx hypertriglyceridemia: association with the improvement of renal mitochondrial ÎČ-oxidation and lipid metabolism in kidney and liver. https://doi.org/10.3390/antiox11112195 | Rat | Kidney |
Fink 2022 FASEB Bioadv | 2022 | Fink BD, Rauckhorst AJ, Taylor EB, Yu L, Sivitz WI (2022) Membrane potential-dependent regulation of mitochondrial complex II by oxaloacetate in interscapular brown adipose tissue. FASEB Bioadv 4:197-210. https://doi.org/10.1096/fba.2021-00137 | Fat | |
Komlodi 2022 BEC | 2022 | KomlĂłdi T, Tretter L (2022) The protonmotive force â not merely membrane potential. Bioenerg Commun 2022.16. https://doi.org/10.26124/bec:2022-0016 | ||
Bouitbir 2022 Int J Mol Sci | 2022 | Bouitbir J, Panajatovic MV, KrĂ€henbĂŒhl S (2022) Mitochondrial toxicity associated with imatinib and sorafenib in isolated rat heart fibers and the cardiomyoblast H9c2 cell line. Int J Mol Sci 23:2282. https://doi.org/10.3390/ijms23042282 | Rat | Heart |
Fink 2021 Pharmacol Res Perspect | 2021 | Fink BD, Yu L, Coppey L, Obrosov A, Shevalye H, Kerns RJ, Yorek MA, Sivitz WI (2021) Effect of mitoquinone on liver metabolism and steatosis in obese and diabetic rats. Pharmacol Res Perspect 9:e00701. | Rat | Liver |
Schmidt 2021 J Biol Chem | 2021 | Schmidt CA, Fisher-Wellman KH, Neufer PD (2021) From OCR and ECAR to energy: perspectives on the design and interpretation of bioenergetics studies. J Biol Chem 207:101140. https://doi.org/10.1016/j.jbc.2021.101140 | ||
Jasz 2021 J Cell Mol Med | 2021 | JĂĄsz DK, SzilĂĄgyi ĂL, Tuboly E, BarĂĄth B, MĂĄrton AR, Varga P, Varga G, Ărces D, MohĂĄcsi Ă, SzabĂł A, BozĂł R, Gömöri K, Görbe A, Boros M, Hartmann P (2021) Reduction in hypoxia-reoxygenation-induced myocardial mitochondrial damage with exogenous methane. https://doi.org/10.1111/jcmm.16498 | Rat | Heart |
Juhaszova 2021 Function (Oxf) | 2021 | Juhaszova M, Kobrinsky E, Zorov DB, Nuss HB, Yaniv Y, Fishbein KW, de Cabo R, Montoliu L, Gabelli SB, Aon MA, Cortassa S, Sollott SJ (2021) ATP synthase K+- and H+-fluxes drive ATP synthesis and enable mitochondrial K+-"uniporter" function: I. Characterization of ion fluxes. Function (Oxf) 3(2):zqab065. doi: 10.1093/function/zqab065 | ||
MiPNet24.08 Safranin Analysis Template | 2020-05-13 | Excel template for safranin data analysis. | ||
MiPNet25.14 TPP Analysis Template | 2020-##-## | Excel template for TPP data analysis. | ||
Gnaiger 2020 BEC MitoPathways | 2020 | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | Human Mouse | Heart Skeletal muscle Fibroblast |
Lozano 2020 Part Fibre Toxicol | 2020 | Lozano O, Silva-Platas C, Chapoy-Villanueva H, PĂ©rez BE, Lees JG, Ramachandra CJA, Contreras-Torres FF, LĂĄzaro-Alfaro A, Luna-Figueroa E, Bernal-RamĂrez J, Gordillo-Galeano A, Benitez A, Oropeza-AlmazĂĄn Y, Castillo EC, Koh PL, Hausenloy DJ, Lim SY, GarcĂa-Rivas G (2020) Amorphous SiO2 nanoparticles promote cardiac dysfunction via the opening of the mitochondrial permeability transition pore in rat heart and human cardiomyocytes. Part Fibre Toxicol 17:15. | Rat Human | Heart |
Wang 2020 J Mol Med (Berl) | 2020 | Wang SY, Zhu Siyu, Wu Jian, Zhang Maomao, Xu Yousheng, Xu Wei, Cui Jinjin, Yu Bo, Cao Wei, Liu Jingjin (2020) Exercise enhances cardiac function by improving mitochondrial dysfunction and maintaining energy homoeostasis in the development of diabetic cardiomyopathy. J Mol Med (Berl) 98:245-61. | Mouse | Heart |
Dolezelova 2020 PLoS Biol | 2020 | DoleĆŸelovĂĄ E, KunzovĂĄ M, Dejung M, Levin M, Panicucci B, Regnault C, Janzen CJ, Barrett MP, Butter F, ZĂkovĂĄ A (2020) Cell-based and multi-omics profiling reveals dynamic metabolic repurposing of mitochondria to drive developmental progression of Trypanosoma brucei. PLoS Biol 18:e3000741. | Protists | |
Oellermann 2020 Sci Rep | 2020 | Oellermann M, Hickey AJR, Fitzgibbon QP, Smith G (2020) Thermal sensitivity links to cellular cardiac decline in three spiny lobsters. Sci Rep 10:202. | Crustaceans | Heart |
Smith 2020 J Biol Chem | 2020 | Smith CD, Schmidt CA, Lin CT, Fisher-Wellman KH, Neufer PD (2020) Flux through mitochondrial redox circuits linked to nicotinamide nucleotide transhydrogenase generates counterbalance changes in energy expenditure. J Biol Chem 295:16207-16. | Mouse | Skeletal muscle |
Cecatto 2020 Mitochondrion | 2020 | Cecatto C, Amaral AU, Wajner A, Wajner SM, Castilho RF, Wajner M (2020) Disturbance of mitochondrial functions associated with permeability transition pore opening induced by cis-5-tetradecenoic and myristic acids in liver of adolescent rats. Mitochondrion 50:1-13. | Rat | Liver Other cell lines |
Hassan 2020 MitoFit Preprint Arch | 2020 | Hassan Hazirah, Gnaiger Erich, Zakaria Fazaine, Makpol Suzana, Abdul Karim Norwahidah (2020) Alterations in mitochondrial respiratory capacity and membrane potential: a link between mitochondrial dysregulation and autism. https://doi.org/10.26124/mitofit:200003 | Human | |
Cecatto 2020 Toxicol In Vitro | 2020 | Cecatto C, Amaral AU, Roginski AC, Castilho RF, Wajner M (2020) Impairment of mitochondrial bioenergetics and permeability transition induction caused by major long-chain fatty acids accumulating in VLCAD deficiency in skeletal muscle as potential pathomechanisms of myopathy. Toxicol In Vitro 62:104665. | Rat | Skeletal muscle |
Charles 2020 Nanomedicine (Lond) | 2020 | Charles C, Cohen-Erez I, Kazaoka B, Melnikov O, Stein DE, Sensenig R, Rapaport H, Orynbayeva Z (2020) Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes. Nanomedicine (Lond) 15:2917-32. | Human | Endothelial;epithelial;mesothelial cell |
Aparicio-Trejo 2020 Free Radic Biol Med | 2020 | Aparicio-Trejo OE, Avila-Rojas SH, Tapia E, Rojas-Morales P, LeĂłn-Contreras JC, MartĂnez-Klimova E, HernĂĄndez-Pando R, SĂĄnchez-Lozada LG, Pedraza-Chaverri J (2020) Chronic impairment of mitochondrial bioenergetics and ÎČ-oxidation promotes experimental AKI-to-CKD transition induced by folic acid. Free Radic Biol Med 154:18-32. | Rat | Kidney |
Fink 2019 FASEB J | 2019 | Fink BD, Yu L, Sivitz WI (2019) Modulation of complex II-energized respiration in muscle, heart, and brown adipose mitochondria by oxaloacetate and complex I electron flow. FASEB J 33:11696-705. | Mouse | Heart Skeletal muscle Fat |
Shuvo 2019 J Bioenerg Biomembr | 2019 | Shuvo SR, Wiens LM, Subramaniam S, Treberg JR, Court DA (2019) Increased reactive oxygen species production and maintenance of membrane potential in VDAC-less Neurospora crassa mitochondria. J Bioenerg Biomembr 51:341-54. | Fungi | |
Spinazzi 2019 Proc Natl Acad Sci U S A | 2019 | Spinazzi M, Radaelli E, Horré K, Arranz AM, Gounko NV, Agostinis P, Maia TM, Impens F, Morais VA, Lopez-Lluch G, Serneels L, Navas P, De Strooper B (2019) PARL deficiency in mouse causes Complex III defects, coenzyme Q depletion, and Leigh-like syndrome. Proc Natl Acad Sci U S A 116:277-86. 10.1073/pnas.1811938116 | Mouse | Nervous system |
Lau 2019 Comp Biochem Physiol B Biochem Mol Biol | 2019 | Lau GY, Milsom WK, Richards JG, Pamenter ME (2019) Heart mitochondria from naked mole-rats (Heterocephalus glaber) are more coupled, but similarly susceptible to anoxia-reoxygenation stress than in laboratory mice (Mus musculus). Comp Biochem Physiol B Biochem Mol Biol 240:110375. | Mouse Other mammals | Heart |
Devaux 2019 Front Physiol | 2019 | Devaux JBL, Hedges CP, Birch N, Herbert N, Renshaw GMC, Hickey AJR (2019) Acidosis maintains the function of brain mitochondria in hypoxia-tolerant triplefin fish: a strategy to survive acute hypoxic exposure? Front Physiol 9:1941. | Fishes | Nervous system |
Esselun 2019 Oxid Med Cell Longev | 2019 | Esselun C, Bruns B, Hagl S, Grewal R, Eckert GP (2019) Differential effects of silibinin A on mitochondrial function in neuronal PC12 and HepG2 liver cells. Oxid Med Cell Longev 2019:1652609. | Human Rat | Nervous system Liver |
Rojas-Morales 2019 Free Radic Biol Med | 2019 | Rojas-Morales P, León-Contreras JC, Aparicio-Trejo OE, Reyes- Ocampo JG, Medina-Campos ON, Jiménez-Osorio AS, Gonzålez-Reyes S, Marquina- Castillo B, Hernåndez-Pando R, Barrera-Oviedo D, Sånchez-Lozada LG, Pedraza-Chaverri J, Tapia E (2019) Fasting reduces oxidative stress, mitochondrial dysfunction and fibrosis induced by renal ischemia-reperfusion injury. Free Radic Biol Med 135:60-67. | Rat | Kidney |
Dilberger 2019 Oxid Med Cell Longev | 2019 | Dilberger B, Baumanns S, Schmitt F, Schmiedl T, Hardt M, Wenzel U, Eckert GP (2019) Mitochondrial oxidative stress impairs energy metabolism and reduces stress resistance and longevity of C. elegans. Oxid Med Cell Longev 2019:6840540. | Caenorhabditis elegans | |
Malyala 2019 PLoS Comput Biol | 2019 | Malyala S, Zhang Y, Strubbe JO, Bazil JN (2019) Calcium phosphate precipitation inhibits mitochondrial energy metabolism. PLoS Comput Biol 15:e1006719. | Guinea pig | Heart |
Hayward 2018 Thesis | 2018 | Hayward L (2018) The effect of anoxia on mitochondrial function in a hibernator (Ictidomys tridecemlineatus). Master's Thesis 57. | Other mammals | Liver |
Fisher-Wellman 2018 Cell Rep | 2018 | Fisher-Wellman KH, Davidson MT, Narowski TM, Lin CT, Koves TR, Muoio DM (2018) Mitochondrial diagnostics: A multiplexed assay platform for comprehensive assessment of mitochondrial energy fluxes. Cell Rep 24:3593-606. | Mouse | Heart Skeletal muscle |
Menezes-Filho 2018 Biochim Biophys Acta Bioenerg | 2018 | Menezes-Filho SL, Amigo I, LuĂ©vano-MartĂnez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. | Mouse | Liver |
McLaughlin 2018 Biochem Biophys Res Commun | 2018 | McLaughlin KL, McClung JM, Fisher-Wellman KH (2018) Bioenergetic consequences of compromised mitochondrial DNA repair in the mouse heart. Biochem Biophys Res Commun 504:742-48. | Mouse | Heart |
Cecatto 2018 FEBS J | 2018 | Cecatto C, Amaral AU, da Silva JC, Wajner A, Schimit MOV, da Silva LHR, Wajner SM, Zanatta A, Castilho RF, Wajner M (2018) Metabolite accumulation in VLCAD deficiency markedly disrupts mitochondrial bioenergetics and Ca2+ homeostasis in the heart. FEBS J 285:1437-55. | Rat | Heart Other cell lines |
Komlodi 2018 J Bioenerg Biomembr | 2018 | KomlĂłdi T, Geibl FF, Sassani M, Ambrus A, Tretter L (2018) Membrane potential and delta pH dependency of reverse electron transport-associated hydrogen peroxide production in brain and heart mitochondria. J Bioenerg Biomembr 50:355-365 | Guinea pig | Heart Nervous system |
Smirnova 2018 Sci Rep | 2018 | Smirnova IA, Ădelroth P, Brzezinski P (2018) Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents. Sci Rep 8:14950. | ||
De Carvalho 2017 Toxicol Research | 2017 | de Carvalho NR, Rodrigues NR, Macedo GE, Boligon AA, de Campos MM, Posser T, Cunha FAB, Coutinho HD, Klamt F, Bristot IJ, Merritt TJS, Franco JL (2017) Eugenia uniflora leaves essential oil promotes mitochondrial dysfunction in Drosophila melanogaster through the inhibition of oxidative phosphorylation. Toxicol Research 6:526-34 . | Drosophila | |
Nogueira 2017 Free Radic Biol Med | 2017 | Nogueira NP, Saraiva FMS, Oliveira MP, Mendonca APM, Inacio JDF, Almeida-Amaral EE, Menna-Barreto RF, Laranja GAT, Lopes Torres EJ, Oliveira MF, Paes MC (2017) Heme modulates Trypanosoma cruzi bioenergetics inducing mitochondrial ROS production. Free Radic Biol Med 108:183-91. | Protists | |
Castellano-Gonzalez 2016 Oncotarget | 2016 | Castellano-GonzĂĄlez G, Pichaud N, Ballard JW, Bessede A, Marcal H, Guillemin GJ (2016) Epigallocatechin-3-gallate induces oxidative phosphorylation by activating cytochrome c oxidase in human cultured neurons and astrocytes. Oncotarget 7:7426-40 | Human | Nervous system |
Moon 2016 J Biol Chem | 2016 | Moon SH, Mancuso DJ, Sims HF, Liu X, Nguyen AL, Yang K, Guan S, Dilthey BG, Jenkins CM, Weinheimer CJ, Kovacs A, Abendschein D, Gross RW (2016) Cardiac myocyte-specific knock-out of calcium-independent phospholipase A2Îł (iPLA2Îł) decreases oxidized fatty acids during ischemia/reperfusion and reduces infarct size. J Biol Chem 291:19687-700. | Mouse | Heart |
Kucera 2015 Oxid Med Cell Longev | 2015 | Kucera O, Mezera V, Moravcova A, Endlicher R, Lotkova H, Drahota Z, Cervinkova Z (2015) In vitro toxicity of epigallocatechin gallate in rat liver mitochondria and hepatocytes. Oxid Med Cell Longev 2015:476180. | Rat | Liver |
Glaser 2014 Thesis | 2014 | Glaser V (2014) Efeitos da hiperglicemia cronica e seus metabolitos, metilglioxal e produtos terminais de glicacao, na fisiologia e dinamica mitochondrial no sistema nervoso central. PhD Thesis 1-117. | Rat | Nervous system Other cell lines |
Casanova 2014 Biochim Biophys Acta | 2014 | Casanova E, Baselga-Escudero L, Ribas-Latre A, Arola-Arnal A, Bladé C, Arola L, Salvadó MJ (2014) Epigallocatechin gallate counteracts oxidative stress in docosahexaenoxic acid-treated myocytes. Biochim Biophys Acta 1837:783-91. | Rat | Skeletal muscle Other cell lines |
Kukat 2014 PLoS Genet | 2014 | Kukat A, Dogan SA, Edgar D, Mourier A, Jacoby C, Maiti P, Mauer J, Becker C, Senft K, Wibom R, Kudin AP, Hultenby K, Flögel U, Rosenkranz S, Ricquier D, Kunz WS, Trifunovic A (2014) Loss of UCP2 attenuates mitochondrial dysfunction without altering ROS production and uncoupling activity. https://doi.org/10.1371/journal.pgen.1004385 | Mouse | Heart |
Pham 2014 Am J Physiol | 2014 | Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499â507. | Rat | Heart |
Gnaiger 2014 MitoPathways | 2014 | Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp. â see 5th edition: Gnaiger 2020 BEC MitoPathways. | Human Mouse | Heart Skeletal muscle Fibroblast |
Felser 2013 Toxicol Sci | 2013 | Felser A, Blum K, Lindinger PW, Bouitbir J, Kraehenbuehl S (2013) Mechanisms of hepatocellular toxicity associated with dronedarone - a comparison to amiodarone. Toxicol Sci 131:480-90. | Human Rat | Liver |
Krako 2013 J Alzheimers Dis | 2013 | Krako N, Magnifico MC, Arese M, Meli G, Forte E, Lecci A, Manca A, GiuffrĂš A, Mastronicola D, Sarti P, Cattaneo A (2013) Characterization of mitochondrial dysfunction in the 7PA2 cell model of Alzheimerâs disease. J Alzheimers Dis 37:747-58. | CHO | |
Duicu 2013 Can J Physiol Pharmacol | 2013 | Duicu OM, Mirica SN, Gheorgheosu DE, Privistirescu AI, Fira-Mladinescu O, Muntean DM (2013) Ageing-induced decrease in cardiac mitochondrial function in healthy rats. Can J Physiol Pharmacol 91:593-600. | Rat | Heart |
Dos Santos 2013 J Bioenerg Biomembr | 2013 | dos Santos RS, Peçanha FL, da-Silva WS (2013) Functional characterization of an uncoupling protein in goldfish white skeletal muscle. J Bioenerg Biomembr 45:243-51. | Fishes | Skeletal muscle |
Volejnikova 2013 FEMS Yeast Res | 2013 | VolejnĂkovĂĄ A, HlouskovĂĄ J, Sigler K, PichovĂĄ A (2013) Vital mitochondrial functions show profound changes during yeast culture ageing. FEMS Yeast Res 13:7-15. | Saccharomyces cerevisiae Fungi | |
Sarti 2013 Int J Mol Sci | 2013 | Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M (2013) New evidence for cross talk between melatonin and mitochondria mediated by a circadian-compatible interaction with nitric oxide. Int J Mol Sci 14:11259-76. | Human | Other cell lines |
Bustamante 2012 Alcohol | 2012 | Bustamante J, Karadayian AG, Lores-Arnaiz S, Cutrera RA (2012) Alterations of motor performance and brain cortex mitochondrial function during ethanol hangover. Alcohol 46:473-9. | Mouse | Nervous system |
Selivanov 2012 PLoS Comput Biol | 2012 | Selivanov VA, Cascante M, Friedman M, Schumaker MF, Trucco M, Votyakova TV (2012) Multistationary and oscillatory modes of free radicals generation by the mitochondrial respiratory chain revealed by a bifurcation analysis. PLoS Comput Biol 8(9):e1002700. doi: 10.1371/journal.pcbi.1002700. | Rat | Nervous system |
Kumari 2012 PLoS One | 2012 | Kumari S, Mehta SL, Li PA (2012) Glutamate induces mitochondrial dynamic imbalance and autophagy activation: preventive effects of selenium. PLoS One 7:e39382. | Mouse | Nervous system Other cell lines |
Albertini 2012 Aging (Albany NY) | 2012 | Albertini E, KozieĆ R, Duerr A, Neuhaus M, Jansen-Duerr P (2012) Cystathionine beta synthase modulates senescence of human endothelial cells. Aging (Albany NY) 4:664-73. | Human | Endothelial;epithelial;mesothelial cell HUVEC |
Leuner 2012 Mol Neurobiol | 2012 | Leuner K, Schulz K, SchĂŒtt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, MĂŒller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204. | Human | Blood cells Lymphocyte |
Brown 2012 Am J Physiol Regul Integr Comp Physiol | 2012 | Brown JC, Chung DJ, Belgrave KR, Staples JF (2012) Mitochondrial metabolic suppression and reactive oxygen species production in liver and skeletal muscle of hibernating thirteen-lined ground squirrels. Am J Physiol Regul Integr Comp Physiol 302:R15-28. | Other mammals | Skeletal muscle Liver |
Selivanov 2011 PLoS Comput Biol | 2011 | Selivanov VA, Votyakova TV, Pivtoraiko VN, Zeak J, Sukhomlin T, Trucco M, Roca J, Cascante M (2011) Reactive oxygen species production by forward and reverse electron fluxes in the mitochondrial respiratory chain. PLoS Comput Biol 7(3):e1001115. doi: 10.1371/journal.pcbi.1001115. | Rat | Nervous system |
Chinopoulos 2011 Methods Mol Biol | 2011 | Chinopoulos C, Zhang SF, Thomas B, Ten V, Starkov AA (2011) Isolation and functional assessment of mitochondria from small amounts of mouse brain tissue. Methods Mol Biol 793:311-24. | Rat | Nervous system |
Brown 2011 Am J Physiol Regul Integr Comp Physiol | 2011 | Brown JC, Chung DJ, Belgrave KR, Staples JF (2011) Mitochondrial metabolic suppression and reactive oxygen species production in liver and skeletal muscle of hibernating thirteen-lined ground squirrels. Am J Physiol Regul Integr Comp Physiol 302:15-28. | Other mammals | Skeletal muscle Liver |
Ziabreva 2010 Glia | 2010 | Ziabreva I, Campbell G, Rist J, Zambonin J, Rorbach J, Wydro MM, Lassmann H, Franklin RJ, Mahad D (2010) Injury and differentiation following inhibition of mitochondrial respiratory chain Complex IV in rat oligodendrocytes. Glia 58:1827-37. | Rat | Nervous system |
Sommer 2010 Eur Respir J | 2010 | Sommer N, Pak O, Schörner S, Derfuss T, Krug A, Gnaiger E, Ghofrani HA, Schermuly RT, Huckstorf C, Seeger W, Grimminger F, Weissmann N (2010) Mitochondrial cytochrome redox states and respiration in acute pulmonary oxygen sensing. https://doi.org/10.1183/09031936.00013809 | Human | Lung;gill Endothelial;epithelial;mesothelial cell |
Dikov 2010 Exp Gerontol | 2010 | Dikov D, Aulbach A, Muster B, Dröse S, Jendrach M, Bereiter-Hahn J (2010) Do UCP2 and mild uncoupling improve longevity? Exp Gerontol 45:586-95. | HeLa Fibroblast HUVEC | |
Stankova 2010 Toxicol In Vitro | 2010 | StaĆkovĂĄ P, KuÄera O, LotkovĂĄ H, RouĆĄar T, Endlicher R, CervinkovĂĄ Z (2010) The toxic effect of thioacetamide on rat liver in vitro. Toxicol In Vitro 24:2097-2103. | Liver | |
Xie 2010 Acta Biochim Pol | 2010 | Xie X, Chowdhury SR, Sangle G, Shen GX (2010) Impact of diabetes-associated lipoproteins on oxygen consumption and mitochondrial enzymes in porcine aortic endothelial cells. Acta Biochim Pol 57:393-8. | Pig | Endothelial;epithelial;mesothelial cell |
Wrzosek 2009 Eur J Pharmacol | 2009 | Wrzosek A, Lukasiak A, Gwozdz P, Malinska D, Kozlovski VI, Szewczyk A, Chlopicki S, Dolowy K (2009) Large-conductance K+ channel opener CGS7184 as a regulator of endothelial cell function. Eur J Pharmacol 602:105-11. | Pig Rat | Heart Endothelial;epithelial;mesothelial cell |
Menna-Barreto 2009 Free Radic Biol Med | 2009 | Menna-Barreto RF, Goncalves RL, Costa EM, Silva RS, Pinto AV, Oliveira MF, de Castro SL (2009) The effects on Trypanosoma cruzi of novel synthetic naphthoquinones are mediated by mitochondrial dysfunction. Free Radic Biol Med 47:644-53. | Protists | |
Soller 2007 Mol Pharmacol | 2007 | Soller M, Dröse S, Brandt U, BrĂŒne B, von Knethen A (2007) Mechanism of Thiazolidinedione-dependent cell death in Jurkat T cells. Mol Pharmacol 71:1535-44. | Heart Other cell lines | |
Labajova 2006 Anal Biochem | 2006 | Labajova A, Vojtiskova A, Krivakova P, Kofranek J, Drahota Z, Houstek J (2006) Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode. Anal Biochem 353:37-42. | Rat | Liver |
Schoenfeld 2004 Biochem J | 2004 | Schönfeld P, Kahlert S, Reiser G (2004) In brain mitochondria the branched-chain fatty acid phytanic acid impairs energy transduction and sensitizes for permeability transition. Biochem J 383:121â28. | Nervous system | |
Huetter 2004 Biochem J | 2004 | HĂŒtter E, Renner K, Pfister G, Stöckl P, Jansen-DĂŒrr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. https://doi.org/10.1042/BJ20040095 | Human | Fibroblast |
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Keywords: Force and membrane potential
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- Fundamental relationships
- » Force
- » Affinity
- » Flux
- » Advancement
- » Advancement per volume
- » Stoichiometric number
- Fundamental relationships
- mt-Membrane potential and protonmotive force
- » Uncoupler
- O2k-Potentiometry
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- » TPP - O2k-Procedures: Tetraphenylphosphonium
- » Specifications: MiPNet15.08 TPP electrode
- » Poster
- » Unspecific binding of TPP+
- » TPP+ inhibitory effect
- O2k-Potentiometry
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- » O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
- » Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
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- O2k-Fluorometry
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MitoPedia concepts: Respiratory state, Recommended, Ergodynamics
MitoPedia methods:
Respirometry,
Fluorometry
MitoPedia topics:
EAGLE
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MitoPedia:NextGen-O2k