Search results

|description=[[File:SUIT-catg NS.jpg|right|300px|NS-pathway control]] ...n for the combination of [[NADH]]-linked substrates (N) and [[succinate]] (S). This physiological substrate combination is required for partial reconsti

:::: PGMS pathway in the LEAK state can be evaluated in the following SUIT protocols: == PGMS_''P'' ==

|info='''B''' '''[[SUIT-003]]''' [[Stadlmann 2006 Cell Biochem Biophys]] ::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling-control protocol]] ce1;ce2SD

|info='''B''' '''[[SUIT-003]]''' [[Stadlmann 2006 Cell Biochem Biophys]] ::: '''[[SUIT protocol pattern]]:''' complex cell [[Coupling-control protocol]] ce1;ce2SD

:::: [[SUIT protocol pattern]]: 1PM;2D;3U;4S;5Rot- ...[[NS-pathway control state| NS]], [[Succinate pathway control state| S]]). SUIT-004 can be extended with the CIV assay module.

|info='''A''' '''[[SUIT-014]]''' |SUIT number=D042_1GM;2D;2c;3P;4S;5U;6Rot;7Ama

...(D001, D002, ..) with links to the specific SUIT pages. Classes of [[SUIT|SUIT protocols]] are explained with coupling and substrate control defined for [ |info=[[MitoPedia: SUIT]]

|abbr=N/S ...ending on the mitochondrial source and various mitochondrial injuries. The S-pathway control state may be selected preferentially as reference state, if

|abbr=Pal+Oct+P+G_OXPHOS+S+Rot_ET ::: '''[[SUIT protocol pattern]]:''' 1PalM;2D;3Oct;4P;5G;6U;7S;8Rot-

|description=[[File:SUIT-catg FNS.jpg|right|300px|F-junction]] ...y control) in combination with [[succinate]] ([[S-pathway control state]]; S- or CII-linked) and one or several fatty acids, which are supplied to feed

|abbr=GM+P+S_OXPHOS+Rot_ET ::: '''[[SUIT protocol pattern]]:''' 1GM;2D;3P;4S;5U;6Rot-

|info='''A: Additivity between the N- and [[Succinate pathway control state| S-pathway]] in the Q-junction''' ::: '''[[SUIT protocol pattern]]:''' 1PM;2D;3G;4S;5U;6Rot-

|info='''B''' - '''[[SUIT-009]]'''- short protocol for respiration in mitochondrial preparations; mt: |SUIT number=D015_1S;2D;3P;4Rot;5Ama

|description=[[File:Ce1;1Dig;1S;2D;2c;3P;4Rot;5Ama.png|520px|SUIT-009]] |info='''B''' - '''[[SUIT-009]]''' - short protocol for mt-respiration in ce: non-permeabilized cells

|abbr= PM+S+Rot ...sub> flux in the NS- pathway control state on isolated mitochondria- '''[[SUIT-031]]'''

|abbr= PM+S+Rot ...</sub> flux in the NS- pathway control state in permeabilized cells- '''[[SUIT-031]]'''

...on=[[File:Pfi;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]] |info=[[SUIT-001]] - '''[[SUIT reference protocol]] Reference protocol RP1 for permeabilized fibers'''

...ns (isolated mitochondria, permabilized cells, and tissue homogenate)-'''[[SUIT-021]]''' |SUIT number=D035_1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama

...IT protocol for mt; mt: isolated mitochondria and tissue homogenate - '''[[SUIT-008]]''' |SUIT number=D026_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd

|abbr=OXPHOS (F+M+P+G+S+Rot) ::: '''[[SUIT protocol pattern]]:''' 1D;2M;3Oct;3c;4M;5P;6G;7S;8Rot-

|description=[[File:1GM;2D;3S;4U;5Rot-.png|400px|SUIT-011]] ::: '''[[SUIT protocol pattern]]:''' 1GM;2D;2c;3S;4U;5Rot-

|info='''A''' pfi: permeabilized fibers - '''[[SUIT-011]]''' |SUIT number=D024_1GM;2D;2c;3S;4U;5Rot;6Ama

|info='''A''' - '''[[SUIT-038]]''' - '''[[Fatty_acid_oxidation_pathway_control_state|F-pathway]] and |SUIT number=D091_1D;2M.1;2H2O;2c;3M.2;3M.5;3M1;3M2;4P;5G;6S10;6S50;7Gp;8U;9Rot;1

|description=[[File:1PGM;2D;3S;4U;5Rot-.png|400px|SUIT-028]] ::: '''[[SUIT protocol pattern]]:''' 1PGM;2D;3S;4U;5Rot-

|abbr=CCP-ce(P) |info='''B''' - [[SUIT-003]]

|abbr=F+G+P+S_OXPHOS+Rot_ET ::: '''[[SUIT protocol pattern]]:''' 1OctM;2D;3G;4P;5S;6U:7Rot-

|abbr=S, SRot |description=[[File:SUIT-catg_S.jpg|right|300px|Succinate]]

...oupling control ([[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET capacity| ''E'']]) with NADH-linked substrates ([[PM-pathway contr ::: '''[[SUIT protocol pattern]]:''' 1PM;2D;2c;3G;4U-

|abbr=PM+S+Rot ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS

::: '''[[SUIT protocol pattern]]:''' 1OctM;2D;3G;4S;5U;6Rot- ...[[FNS]] in [[Oxidative phosphorylation|OXPHOS state]] and of [[FNS]] and [[S]] in [[ET capacity| ET state]] is evaluated.

...on=[[File:Mt;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd .png|400px|SUIT-RP1]] |info=[[SUIT-001]] - '''[[SUIT reference protocol]] Reference protocol RP1 for isolated mitochondria, tiss

...File:ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|600px|SUIT-RP1]] |info='''A''' - '''[[SUIT-001]]''' - '''[[SUIT reference protocol]] Reference protocol RP1 for living cells (ce, ROUTINE r

...le:ce1;1Dig;1PM;2D;2c;3U;4G;5S;(6Oct);7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1 for PBMC and PLT]] |info='''A''' - '''[[SUIT-001]]''' - '''[[SUIT reference protocol]] Reference protocol RP1 for living PBMC and PLT cells (

|info='''A''' - '''[[SUIT-039]]''' - '''[[Fatty_acid_oxidation_pathway_control_state|F-pathway]] and |SUIT number=D092_1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8U;9Rot;10Ama

|info='''A''' - '''[[SUIT-039]]''' - '''[[Fatty_acid_oxidation_pathway_control_state|F-pathway]] and |SUIT number=D093_1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8U;9Rot;10Ama

...='''[[SUIT-004]]''' - Short protocol linked to [[SUIT-001 O2 pfi D002]] '''SUIT RP1''' (human skeletal muscle) |SUIT number=D010_1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8Azd

...Pfi;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px|SUIT-RP2]] |info='''A''' -'''[[SUIT-002]]''' - '''[[SUIT reference protocol]] Reference protocol RP2 for permeabilized fibers (pfi)'

|info='''A: for cells-permeabilized cells''' [[Lemieux 2017 Sci Rep]] - '''[[SUIT-008]]''' |SUIT number=D025_ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd

|abbr=SUIT-catg |description=[[File:SUIT-catg_MitoPathway types.jpg|right|200px]]

|abbr= PM+S+Rot ...in the N-,NS- and S- pathway control state on isolated mitochondria- '''[[SUIT-031]]'''

|info='''A''' - [[Lemieux 2017 Sci Rep]] - '''[[SUIT-008]]'''- O2 pfi D014 protocol was used a a reference protocol in [[MitoEAG |SUIT number=D014_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd

|info='''A''' - '''[[SUIT-037]]''' - '''[[Fatty_acid_oxidation_pathway_control_state|F-pathway]] and |SUIT number=D090_1D;2M.1;3Oct;3c;4M.2;4M.5;4M1;4M2;5P;6G;7S10;7S50;8Gp;9U;10Rot;

:::: GMS pathway in the LEAK state can be evaluated in the following SUIT protocols: == GMS_''P''==

|info='''A''' - '''[[SUIT-036]]''' - '''[[Fatty_acid_oxidation_pathway_control_state|F-pathway]] and |SUIT number=D089_1D;2M.1;3Pal;3c;4M.2;4M.5;4M1;4M2;5P;6G;7S10;7S50;8Gp;9U;10Rot;

|description=[[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp-.png|400px|SUIT-001]] |mitopedia concept=MiP concept, SUIT protocol, Recommended

|description=[[File:SUIT-catg N.jpg|right|300px|N-junction]] ...tabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the N-pathway control state can be further suppressed

...Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px|SUIT-RP2]] |info='''A''' - '''[[SUIT-002]]''' - '''[[SUIT reference protocol]] Reference protocol RP2 for living cells (ce, ROUTINE r

|abbr=F+G+S+Rot_OXPHOS+Omy ::: '''[[SUIT protocol pattern]]:''' 1OctM;2D;3G;4S;5Rot;6Omy;7U-

|info='''A''' - '''[[SUIT-002]]''' - '''[[SUIT reference protocol]] Reference protocol RP2 for isolated mitochondria, tiss |SUIT number=D005_mt;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

|abbr= PM+S+Rot ...-redox state in the NS- pathway control state on permeabilized cells- '''[[SUIT-031]]'''

...Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px|SUIT-RP2 for PBMC and PLT]] |info='''A''' - '''[[SUIT-002]]''' - '''[[SUIT reference protocol]] Reference protocol RP2 for for living PBMC and PLT cel

|info='''A''' pfi: permeabilized fibers- '''[[SUIT-016]]''' |SUIT number=D044_1OctM;2D;3G;4S;5Rot;6Omy;7U;8Ama

...s (isolated mitochondria, permeabilized cells, and tissue homogenate)-'''[[SUIT-020]]''' |SUIT number=D032_1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8Ama

|abbr=CCP-ce S permeability test |info='''B''': CCP(P) and cell membrane permeability test with succinate - [[SUIT-003]]

...sub> (AmR) in ce: non-permeabilized cells, pce: permeabilized cells -'''[[SUIT-009]]''' |SUIT number=D019_ce1;1Dig;1S;2D;3P;4Rot;5Ama

::: '''[[SUIT protocol pattern]]:'''1S;2D;3P;4Rot- ...related H₂O₂ production in the LEAK state. Pyruvate (P) promotes[[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usu

::: '''[[SUIT protocol pattern]]:''' 1D;2M;3Oct;4M;5P;6G;7S;8Gp;9U;10Rot- ...pathway control state|SGp]]) can be evaluated by using this SUIT protocol. SUIT-002 can be extended with the CIV assay module.

...(isolated mitochondria, permeabilized cells, and tissue homogenate)- '''[[SUIT-020]]''' |SUIT number=D033_1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama

(S, resolution respirometry using a substrate uncoupler inhibitor titration (SUIT) protocol.

|title=Development of a SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. |info=[[MiPNet21.06 SUIT RP]]

|title=Boushel RC, Gnaiger E, Schjerling P, Skovbro M, Kraunsoee R, Dela F (2007) Patients with Type 2 diabetes have n |authors=Boushel RC, Gnaiger Erich, Schjerling P, Skovbro M, Kraunsoee R, Dela F

|abbr=OXPHOS (GM+S+Rot+Omy) ::: '''[[SUIT protocol pattern]]:''' 1GM;2D;3S;4Rot;5Omy;6U;-

::: '''[[SUIT protocol pattern]]:''' 1X;2D;3Omy;4U;5Ama ...city| ''E'']] or [[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[LEAK respiration|''L''(Omy)]]-[[ET capacity| ''E'']]) at a defined [[

|description=[[File:1S;2D;3P;4Rot;5Ama.png|400px|SUIT-009]] ...t: isolated mitochondria, tissue homogenate and permeabilized cells - '''[[SUIT-009]]'''short protocol for H₂O₂ (AmR) for isolated mi

|title=Larsen S, Kristensen JM, Stride N, Wojtaszewski JF, Helge JW, Dela F (2011) Skeletal |authors=Larsen S, Kristensen JM, Stride N, Wojtaszewski JF, Helge JW, Dela F

SUIT reference protocol for comparative mitochondrial physiology. |authors=Doerrier C, Neves P, Passrugger M, Krumschnabel G, Gnaiger E

...s (isolated mitochondria, permeabilized cells, and tissue homogenate)-'''[[SUIT-021]]''' |SUIT number=D036_1GM;2D;3S;4Rot;5Omy;6U;7Ama

...le=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. |abstract=Doerrier C, Sumbalova Z, Gnaiger (2016) SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. Mit

...=[[File:DoerrierC.JPG|left|90px]] Substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. ...rial physiology. In conclusion, an optimal design and comprehension of the SUIT protocols is crucial for a proper evaluation of mitochondrial function in h

...(''P''<0.01), with the same trend in ET_H and ST_H (''P''<0.1). The limitation of flux by the phosphorylation system was diminished == SUIT protocol ==

== SUIT protocols == [[File:1PM;2D;3G;3c;4S;5U;6Rot-.jpg|300px]] [[SUIT-008 O2 pfi D014 |1PM;2D;3G;4S;5U;6Rot-]]

...ondrial disease. A standardized substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocol [1,2] was used for measuring respiration of permeabilized fibro ...ic sensitivity than complex specific O₂ fluxes. The established SUIT protocol is an important tool in the diagnostic process. Therefore, we reco

|info=[[File:PaulCoen.jpg |150px|right|Coen P]] [[Laner 2014 Mitochondr Physiol Network MiP2014|Mitochondr Physiol Networ |authors=Coen P, DiStefano G, Tanner C, Helbling NL, Dubis G, Toledo F, Xie H, Eid G, Houma

...espiration from permeabilized muscle fibers and PBMCs using an established SUIT-protocol. <br> ...OS capacity correlated negatively with diabetes disease duration (r=-0.50, p=0.02). These results suggest that there are no sex-specific differences wit

...he anion '''malate^2-, M''', with p''K''_a1 = 3.40 and p''K''_a2 = 5.20. L-Malate is formed from fumarate in the [[TCA cyc ...verestimated due to a contribution of NADH-linked respiration, F(N) (see [[SUIT-002]]).

...co Adam J, Zilhaver Philip T, Whitcomb Luke A, Fresa Kyle J, Izon Cheyanne S, Gonzalez-Franquesa Alba, Dometita Crystal, Irving Brian A, Garcia-Roves Pa ...n ''J''_O₂, revealing a complex interaction of N- and S-pathway substrate oxidation on ''J''_O₂.

|title=Optimizing strategies on the malate concentration in SUIT protocols. ...) cycle will, besides stimulating NADH production, also produce succinate (S) feeding CII. The extent of the contribution of CII to the overall respirat

...mitochondria and, in a forward step, (2) investigated whether changes in ''p''₅₀ reflect inhibition of COX if used as a diagnostic test. ...amber amperometrically (WPI). In another set of experiments, mitochondrial p₅₀ was estimated to detect partial inhibition of COX by an unknow

...the males had higher OXPHOS_GMS (''P''<0.05) and ET capacity (''P''<0.05) than the females. In the liver, no significant differences were obs # Iossa S, Lionetti L, Mollica MP, Crescenzo R, Botta M, Barletta A, Liverini G (2003

...as well as of the NADH-linked and succinate (S)-pathways separately (F, N, S) and in combination (FN, FS, FNS, NS). ...gests an unspecific effect of on mitochondrial respiration involving N-and S-pathways and raises caution in its application as specific inhibitor of FAO

..., Damsgaard R, Hagen C, Díez-Sánchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitocho ..., Damsgaard R, Hagen C, Diez-Sanchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet Jose AL, Christiansen M, Helge JW, Saltin Bengt

|title=Stadlmann S, Renner K, Pollheimer J, Moser PL, Zeimet AG, Offner FA, Gnaiger E (2006) P |authors=Stadlmann S, Renner K, Pollheimer J, Moser PL, Zeimet AG, Offner FA, Gnaiger Erich

|abbr=S ...nion '''succinate^2-, S''', with ''p''K_a1 = 4.2 and ''p''K_a2 = 5.6. Succinate is formed in the [[TCA cycle]], and is a s

|authors=Doerrier C, Gama-Perez P, Garcia-Roves P, Gnaiger E ...y]] in pfi from soleus muscle from ''N''=15 C57BL6/J male mice using the [[SUIT-008 O2 pfi D014]] protocol (Fig. 1) [2]. Experiments were performed in para

...sacrifice. Quadriceps muscles were used for the respiration measurements. SUIT protocols were performed in permeabilized fiber bundles using either Comple ...to further improve coupling efficiency in the ''Nampt''Tg mice as well (''P''=0.107; Fig. 1B).

|authors=Garcia-Roves Pablo M, Chabi B, Doerrier C, Dubouchaud H, Grefte S, Irving B, Ost M, Pesta D ...skeletal muscle fibers. The substrates, uncouplers, inhibitors titration (SUIT) protocol that we are assessing is represented in Figure 1. Finally, we wil

...ndrial physiology [2]. A rationale for categorizing PCS helps in selecting SUIT protocols according to the specific research question or diagnostic aim, an ...respiratory capacity [2-5]. Analysis of combined NS- versus single N- and S-pathway capacities yields information on pathway interactions and channelin

...edia:_SUIT |SUIT protocols]] - The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions. File:SchmittS.jpg|'''[[Schmitt S|Sabine Schmitt]]''', PhD, Oroboros Instruments - ''Mitochondrial Detective'

...kinetic O₂ saturation. In diagnostic experiments, increasing ''p₅₀'' values correlated well with decreasing CIV activity, as meas Mitochondrial ''p₅₀'' measurement is a quick, highly accurate and sensitive way fo

...bition with sodium azide to correct for chemical background reactions. The SUIT protocol, including different substance combinations, allows measurement of ...on of succinate (S) to quantify CI+&II-linked OXPHOS capacity, CI&II_''P''; stepwise uncoupling with FCCP (F1-F4) to obtain CI&II-linked ET ca

|Demo: Instrumental quality control 1. Introduction to SUIT protocols RP1&RP2 |SUIT protocols

...(Oroboros Ltd.), according to the Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocol adapted to measure both complex I and complex II-dependent respir ...: RCR - 4.23±0.16 vs 6.65±0.3. (p<0.001) and R/E - 0.42±0.04 vs 0.31±0.01 (p<0.05), respectively.

| SUIT-001_O2_mt_D001 | {{Template:SUIT text D001}}

...ria E, Casado M, Fernandez-Ortiz M, Acuna-Castroviejo D, Villena J, Grefte S, Keijer J, O'Brien KA, Sowton A, Murray AJ, Campbell MD, Marcinek DJ ...7BL/6J mice aged 14-16 weeks. The substrate-uncoupler-inhibitor titration (SUIT) protocol used in the study is represented in Figure 1 [1]. The presentatio

...rgaard SD, Chroeis KM, Gonzalez-Franquesa A, Coen P, Goodpaster BH, Larsen S, Gnaiger E ...described in the substrate-uncoupler-inhibitor titration (SUIT) protocol [[SUIT-008 O2 pfi D014]] (Fig. 1) [7]. Results of this study will be presented dur

Complementary to the concept of [[flux control ratio]]s and analogous to [[elasticity|elasticities]] of [[metabolic control analysi ...en state relative to respiratory flux in a reference state. Whereas ''FCR''s express various respiratory states relative to a common refrence state, ''j

|authors=Nemec M, Maderova D, Krumpolec P, Sumbalova Z, Vician M, Slobodova L, Schoen M, Krssak M, Ukropcova B, Ukrop ...tocols RP1 and RP2 [4] were used to evaluate oxygen consumption rate (pmol s^-1mg^-1 tissue wet weight) in saponin permabilized musc

...he best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coachi | '''[[UK London Filippi C]]''' - King's College Hospital, London (UK)

...n respirometry (HRR) and multiple substrate-uncoupler-inhibitor titration (SUIT) protocols were applied to study mitochondrial respiration in triceps brach |pathways=F, N, S, NS, ROX

...eated measures one-way ANOVA and post-hoc pairwise comparisons using Tukey’s correction for multiple comparisons. ...lux, respectively. PGM_L was strongly affected by temperature (p<0.0001), with a maximum value of 49.5 ± 8.8 at 44°C. No other respirator

...de promotors on mitochondrial bioenergetics in a murine model of Alzheimer's disease. '''Bioblast 2022: BEC Inaugural Conference.''' In: https://doi.org |authors=Irving Brian A, Stampley J, Quiriarte H, Wigger Z, Stephens J, Soto P, Allerton TA

|authors=Ukropec J, Nemec M, Maderova D, Tirpakova V, Krumpolec P, Slobodova L, Schoen M, Sumbalova Z, Cvecka J, Vician M, Sedliak M, Ukropco ...respirometry applying SUIT protocols RP1 and RP2. Oxygen consumption (pmol.s^-1.mg^-1 tissue wet weight) was evaluated in saponin-pe

...ich limits mitochondrial O₂ consumption rate to half-maximum (''p''₅₀) has implications in a wide range of biomedical research top ...g repeated anoxic transitions in each respiratory state in the course of [[SUIT]] protocols.

...rgaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respir ...rgaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger Erich, Garcia-Roves PM

|title=Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Increased OXPHOS capacity after co |authors=Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E

|title=Chicco AJ, Le CH, Schlater A, Kanatous S (2013) Comparative muscle mitochondrial physiology of the northern elephant |authors=Chicco AJ, Le CH, Schlater A, Kanatous S

...hway (S). Superimposed oxygraph traces (mass-specific oxygen flux [pmol O2∙s^-1∙mg^-1 ''m''_w]) from parallel measuremen ...onal terms to account for the network of metabolic interactions in complex SUIT protols with stepwise modulation of coupling and substrate control. A regul

...owton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wuest R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM ...it following the substrate-uncoupler-inhibitor titration (SUIT) protocol [[SUIT-008_O2_pfi_D014]] represented in Figure1 [1]. Chemicals were provided by ea

...compounds are prepared by just adding the solvent instead of adjusting it's volume. For example, this approach is useful if the substance is very toxic '''Strategy:''' P, Gp and TMPD were directly weighted in 2 mL Eppendorf tubes. Azide turned o

...hibitors (SUIT protocol) [6]. After signal stabilization, the experimental SUIT protocol was performed by sequential addition of pyruvate (5 mM), malate (2 |diseases=Aging;senescence, Parkinson's

...n of respiratory flux is 1 pmol∙s^-­1∙cm^-­3 (0.001 µM∙s^-­1) and the limit of detection of oxygen concentration extends ...tep increase in the final concentration of 0.5 μM FCCP at intervals of 120 s. Maximum noncoupled flux (capacity of the Electron transfer-pathway, [[ET-c

...d cells, living cells). Optimized substrate-uncoupler-inhibitor titration (SUIT) protocols offer a powerful tool for comprehensive mitochondrial physiology ...ame of collaborative Open-Innovation (NextGen-O2k with Q-redox sensor, NAD(P)H autofluorescence and PhotoBiology module) to promote quality and reproduc

...preparation of chemicals used in Substrate-Uncoupler-Inhibitor Ttitration (SUIT) protocols. ...r expensive, the solvent is added instead of adjusted. For some compounds (P, Gp, Glu, Azd) the correct volume to be added was experimentally determined

# Israelson A, Arbel N, da Cruz S, Ilieva H, Yamanaka K, Shoshan-Barmatz V, Cleveland DW (2010) Misfolded mut # Magrì A, Reina S, De Pinto V (2018) VDAC1 as pharmacological target in cancer and neurodegen

...he best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coachi | '''DE Jena Coldewey S''' - Jena University Hospital, Jena (DE)

...d as ''Rox''-corrected rates, in contrast to total rates ''R''´, ''L''´, ''P''´ and ''E''´. When expressing ''Rox'' as a fraction of ET capacity ([[fl ...ted for ROX. ''Apparent'' fluxes (not corrected for ROX; ''R''´, ''L''´, ''P''´ and ''E''´) places the terminology into the conceptual context of phys

...al service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. :::: Early stage researchers (ESR´s) during their Marie Skłodowska-Curie project - [[TRANSMIT]] or [[REMIX]] w

...ells were permeabilized to ensure access of external substrates. Following SUIT was performed: ...strate''' || '''P''' || '''M''' || '''Dig''' || '''ADP''' || '''G''' || '''S''' || '''u''' || '''Rot''' || '''Ama'''

|description=[[File:SUIT-catg FNSGpCIV.jpg|right|400px]] ...te|ET-pathway competent states]], including oxygen supply. [[Categories of SUIT protocols]] are defined according to mitochondrial ET-pathway states.

::::* Díaz-Ruiz R, Avéret N, Araiza D, Pinson B, Uribe-Carvajal S, Devin A, Rigoulet M (2008) Mitochondrial oxidative phosphorylation is regu ::::* Vadlakonda L, Dash A, Pasupuleti M, Anil Kumar K, Reddanna P (2013) Did we get Pasteur, Warburg, and Crabtree on a right note? Front Onc

...owton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wuest R, Dayanidhi S ...7BL/6J mice aged 14-16 weeks. The substrate-uncoupler-inhibitor titration (SUIT) protocol used in the study is represented in Figure 1 [1]. The presentatio

...harská J, Rausová Z, Palacka P, Kovalčíková E, Takácsová T, Mojto V, Navas P, López-Lluch G, Gvozdjáková A (2022) Ubiquinol supplementation accelerat ...harska J, Rausova Z, Palacka P, Kovalcikova E, Takacsova T, Mojto V, Navas P, Lopez-Lluch G, Gvozdjakova A

== SUIT protocols and terminology == :::: SUIT protocols: nomenclature consistent with concept of database'''

...='''Fatty acid oxidation''' is a multi-step process by which [[fatty acid]]s are broken down in [[β-oxidation]] to generate acetyl-CoA, NADH and FADH<s ...) with fatty acid-free [[Bovine serum albumine]] <ref> Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control

== HRR: high-resolution respirometry and SUIT protocols == ::::» Introduction to harmonized reference protocols 1 and 2 (SUIT-001 and 002) for mitochondrial preparation.

...glass cell homogenizer and series of centrifugation steps. We utilized two SUIT protocols to examine respiration in high-resolution: RP2 and a previously p |diseases=Alzheimer's

...mg) were permeabilized by saponin [1]. Using SUIT protocol (G, M, P, D, c, S, Rot, Ama, As+Tm, Azd) standard respiratory states (LEAK, OXPHOS, and ROX), |pathways=N, S, CIV, NS, ROX

...Siluriformes, Perciformes (Cichlidae). The results obtained with a basic [[SUIT protocol]] will be compared with mitochondrial respiratory control patterns Day 2: SUIT protocol for comparative mitochondrial physiology - a pilot experiment with

...(CI), and CI&II-linked OXPHOS and ET capacity were determined in a single SUIT protocol. Additionally, neutrophils, monocytes and lymphocytes (inflammator ...d time of recovery on human platelet metabolism. We found a significant (''p''<0,05) relationship between BMI and CI/CI&II ratio, whereas age and traini

...of TBARS negatively correlated with CoQ₁₀ in plasma (r=-0.354, P<0.02). |pathways=F, N, S, Gp, CIV, NS, ROX

...ented. Skeletal muscle tissue processing for high-resolution respirometry, SUIT protocols and integration of data results will be emphasized during this ta ...reased biogenesis of mitochondria in skeletal muscle. Proc Natl Acad Sci U S A 104:10709-13.

...O₂ || [[ATP yield]] || ''Y''_P»/O₂ || 1 || P»/O₂ ratio measured in any respiratory state; Section 1.6; Eq. 3 ...'' and subscript ''X'' signal 'per count', subscript ce signals the entity s=ce; in a context restricted to cells or molecules or a particular organism

...s in the absence of adenylates (LEAK, ''L''), at saturating ADP (OXPHOS, ''P''), and in uncoupler titrations (ET capacity, ''E''). |pathways=N, S, NS

...n tissues under physiological or pathological conditions, we established a SUIT protocol for the analysis of oxidative phosphorylation (OXPHOS) and electro ...per tissue mass or as CIV/CI&II_''E'' flux control ratios. The ''P/E'' coupling control ratio (CI&II), an index of the limitation of OXPHOS ca

...he best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coachi | '''[[AT Innsbruck Schneeberger S]]''' - Medical University of Innsbruck, AT

...s Oxygraph-2k applying standard substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocols for permeabilized cells in [[MiR05]]. For HRR with intact cell ...compared to cells maintained in culture, but CI''_P'', CI&II_P, and CI&II_E were reduced. CI<small>&</small>II-linked OXPH

...fer from reduced substrates to oxygen drives the mt-membrane potential, Δ''p''_mt, through the proton pumps of respiratory Complexes CI, CIII ...ies of mt-preparations include measurement of respiration, ATP production (P»), mt-membrane potential and ROS production, which require substrates to b

''' How did Peter Mitchell coin Δp ? ''' ...[pH]] stems from the term ''potentia hydrogenii''. What is the message of Δp (Gnaiger 2020)?

...Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wüst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM (2019) Generating reference values ...owton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wuest R, Dayanidhi S, Gnaiger Erich, Doerrier C, Garcia-Roves PM

...grating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols in mt-respiration medium MiR06Cr. Two substrate-uncoupler-inhibi ...ng 200 pmol∙^-11∙mg^-1 Ww [3], compared to 153±19 pmol∙s^-1∙mg^-1 Ww in competitive racing horses [4].

...community with presentations from and a panel discussion with the project's Key Opinion Leaders. | 16:10 || [[File:SUIT-catg FNSGp.jpg|left|200px|Q-junction|link=Komlodi 2021 MitoFit Q]] Simultan

...OXPHOS capacity. We developed the substrate-uncoupler-inhibitor titration (SUIT) protocol RP2 for reliable FAO evaluation by high-resolution respirometry ( ...Nagel WO, Morris HP (1980) Mitochondrial malic enzymes. Mitochondrial NAD(P)+-dependent malic enzyme activity and malate-dependent pyruvate formation a

...grating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols in mt-respiration medium MiR06Cr. Two substrate-uncoupler-inhibi ...ng 200 pmol∙s^-1∙mg^-1 Ww [3], compared to 153±19 pmol∙s^-1∙mg^-1 Ww in competitive racing horses [4].

...grating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols in mt-respiration medium MiR06Cr. Two substrate-uncoupler-inhibi ...ng 200 pmol∙s^-1∙mg^-1 Ww [3], compared to 153±19 pmol∙s^-1∙mg^-1 Ww in competitive racing horses [4].

...print website. If you prefer to submit comments in the format of a referee's evaluation rather than a contribution as a coauthor, I will be glad to dist ...to pathway-related nomenclature instead of enzyme-linked terminology (N/NS/S versus CI/CI&II/CII)

..., Damsgaard R, Hagen C, Díez-Sánchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet J, Christiansen M, Helge JW, Saltin B, Boushel R (2013 ..., Damsgaard R, Hagen C, Diez-Sanchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet J, Christiansen M, Helge JW, Saltin B, Boushel RC,

..., Ca²⁺) and [[O2k-NO Amp-Module|amperometry]] (NO, H₂S). ...thways|MitoPathways]]Specific [[substrate-uncoupler-inhibitor titration]] (SUIT) protocols are applied in studies of cell respiration or mitochondrial acti

:::* [[Platen P|Petra Platen]] (Prof Dr Ruhr University Bochum, Sportmedizin) :::* [[Malacrida S|Sandro Malacrida]] (EURAC Bolzano/Bozen - Center for Biomedicine)

...are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions. Furthermore, this workshop c :::::» Chapters 4-5: NADH-linked pathways through Complex CI and S-pathway through Complex CII (estimated reading time 30 min – 1 h)

...try (Oroboros Oxygraph-2k) with a SUIT protocol. Respiration flux (pmol O2 s^-1 mg^-1) was determined using DatLab 6.0 and statistic |pathways=N, S

:::* Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric ...ke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia toler

...s, and various sequences of substrate, inhibitor and uncoupler titrations (SUIT) to generate different respiratory states. Respirometry indices measured i :::#Brealey D, Brand M, Hargreaves I, Heales S, Land J, Smolenski R, Davies NA, Cooper CE, Singer M (2002) Association bet

...ion of protons. 2. From Einstein’s diffusion equation on gradients to Fick’s law on compartments.'' - [[Gnaiger 2018 MiPschool Tromso A2]] + To express both in identical [[motive unit]]s [MU]

...h-resolution respirometry using substrate-uncoupler-inhibitor titration ([[SUIT]]) protocols on permeabilized fibers [1,2]. The patient performed an in-viv ...an unsuspicious PCr recovery time (''t''=40 s). This is in line with a ''[[P/E]]'' ratio of ~0.9 and normal coupling control of mitochondrial respiratio

...'''[[Dayanidhi Sudarshan]]''', O2k-Network Lab: [[US IL Chicago Dayanidhi S]] File:IOC115 (5).jpg|Pedro explaining the steps in the SUIT protocols

...ing of oxygen concentration, respiration and hydrogen peroxide production. SUIT protocol application and interpretation, as well as calibration and instrum File:MeszarosA.JPG|'''[[Meszaros A|András Mészáros]]''', MD., PhD., Oroboros Instruments - Chief research officer

...design by combining the technical advantages of closed and [[open system]]s approaches. |info=[[BEC 2020.1]], [[Gnaiger 2000 Proc Natl Acad Sci U S A]], [[Gnaiger 2001 Respir Physiol]], [[Harrison 2015 J Appl Physiol]]

:::: [[File:DL 2017-02-27 PS7 02.jpg|left|450px |SUIT protocol]] :::# Karabatsiakis A, Böck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peri

...are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions. Furthermore, this workshop c |[[Dantas Wagner S]]

::::# Substrate-uncoupler-inhibitor-titration (SUIT) protocols with the new software [[MiPNet21.16 DatLab 7 Innovations |DatLab File:IOC131 21.JPG|120px|[[Rodhiah S |Siti Nor Rodhiah Rosaidee]], [[MY Kuala Lumpur Abdul Karim N]]

...ation, respiration, hydrogen peroxide production or mt-membrane potential. SUIT protocols, calibration and instrumental service are covered as well. :::: Early stage researchers (ESR´s) during their Marie Skłodowska-Curie project - [[TRACT]] or [[TRANSMIT]] a

[[File:SUIT-nomenclature.jpg|right|400px|link= MitoPedia: SUIT|SUIT protocols]] === A1: Diagnostic SUIT protocols of mitochondrial fitness ===

:::: Several substances, typically used in SUIT protocols, may influence the fluorescence signal of safranin when injected ::::* [[Succinate]] (S)

::::» [[P/E]] ::::» [[SUIT protocol]]s

| [[MiPNet21.06 SUIT RP]], [[Makrecka-Kuka 2015 Biomolecules]] Data discussion, SUIT protocol interpretation, summary.

....mitophysiology.org/?MiP-summerschool2007 MiPsummer school 2007], advanced SUIT protocols for sequential activation particularly of CI-, CI<small>&</small> ...on the [[O2k-Core]], developed in cooperation with WGT-Elektronik ([[Gradl P|Philipp Gradl]]) in the framework of the K-Regio Project ''[[MitoCom_O2k-Fl

...and to discuss scientific talks. I think it was really useful to see the SUIT protocols, the POS services, the background and other protocols related to ...are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions. Furthermore, this workshop c

at the Children’s Hospital Research Institue # '''[[Breton Sophie]]''', [[CA_Montreal_Breton S]]: Department of Biological Sciences, University of Montréal. - Mitochondr

...et new, hopefully long and fruitful collaborations.'' - [[Crisostomo L |Luís Crisóstomo, MSc, PhD Student]], Porto, PT. ...eos taken during the meeting as well. Hope to see you all soon.'' - [[Iyer S| Shilpa Iyer]], Fayetteville , AR, US

...s are available as DL-Protocols.The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Furthermore, this workshop c | '''[[UK Loughborough Bailey S]]''' - Loughborough University, Loughborough (UK)

...lly informative, I learned alot and the team is super helpful. Although it's a full programme the walk and talk or run and fun were great to have a brea ...and to discuss scientific talks. I think it was really useful to see the SUIT protocols, the POS services, the background and other protocols related to

...ing an experiment due to AmR consumption and dilution by titrations in a [[SUIT protocol]]. Check for any potential effects of the experimental AmR concent :::: [[Horseradish_peroxidase|Horse radish peroxidase]] (Sigma-Aldrich P 8250 - 5 KU): Prepare a stock solution with 500 U HRP/mL in MiR05 or MiR05C

...to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeab - »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>

:::: TRANSMIT project manager: [[Paterlini S|Serena Paterlini]] - [http://www.unibo.it/en/ Alma Mater Studiorum – Univ | [[Sarlak S | Saharnaz Sarlak]]

...o mitochondrial respiration? In: Schuster S, Rigoulet M, Ouhabi R, Mazat J-P (eds) Modern Trends in Biothermokinetics. Plenum Press, New York, London:28 # [[Gnaiger 2000 Life in the Cold|Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000b) Mitochondria in th

...ersus BMI - an issue of mitObesity and gender medicine in our modern world's obesogenic environment - ''More details:'' » [[Body fat excess]] ::::* 16:00-17:00 [[Schmitt S|Sabine Schmitt]]: The antibiotic Tigecycline impairs mitochondrial ATP prod

...in conjunction with multiple [[substrate-uncoupler-inhibitor titration]] (SUIT) protocols, respirometric studies of human and animal tissue biopsies impro :::* [[Lemieux_2011_Int_J_Biochem_Cell_Biol| Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control

:::: High Altitude Training Center Kühtai, Kuehtai (Tyrol, Austria), 2020 m a.s.l. (42 min from Innsbruck). ::::* Activities at the participant's own risk. Sport programme is offered in collaboration with the Institute of

...ration, ''L'', is higher than the LEAK component of [[OXPHOS capacity]], ''P''. The conditions for measurement and expression of respiration vary ([[oxy ...of ATP is added in the absence of ADP ([[Gnaiger 2000 Proc Natl Acad Sci U S A]]).

...o mitochondrial respiration? In: Schuster S, Rigoulet M, Ouhabi R, Mazat J-P (eds) Modern Trends in Biothermokinetics. Plenum Press, New York, London:28 :::# Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000b) Mitochondria in th

...concentration, mitochondrial respiration and hydrogen peroxide production. SUIT protocols, calibration and instrumental service are covered as well. | '''US TN Memphis Alway S''' - University of Tennessee Health Science Center, Memphis (US)

...MiP school would be nice, but may prove to be logistically difficult. STSM’s would be more appropriate for this. File:Ali S photo.jpg|'''[[Ali SS|Ali Sameh]]''' (57357 Children’s Cancer Hospital, EG)

|'''[[AT Innsbruck Jansen-Duerr P]]''': University of Innsbruck (AT) |[[Barth S|Barth Sonja]]

..., Konopka P, Paszko A, Citko A, Szczerbinski K, Goscik J, Gorska M, Larsen S, Kretowski A (2021) The response of mitochondrial respiration and quantity :from the O2k-Network »[[DK Copenhagen Larsen S]]«

...Čižmárová Beata, Danila Maria-Daniela*, Doerrier Carolina*, Eckert Gunter P*, Ehinger Johannes K, Elmer Eskil*, Garcia-Souza Luiz F*, Gnaiger Erich*, H ...n, Cizmarova Beata, Danila Maria-Daniela, Doerrier Carolina, Eckert Gunter P, Ehinger Johannes K, Elmer Eskil, Garcia-Souza Luiz F, Gnaiger Erich, Hoppe

...ibitor rotenone is widely used to develop experimental models of Parkinson’s disease, Gangly et al examined if rotenone is an inducer of ferroptosis. <b * Ganguly U, Bir A, Chakrabarti S (2022) Cytotoxicity of mitochondrial Complex I inhibitor rotenone: a comple

| [[Gama Pérez P|Gama Pérez Pau]] | [[Larsen S]] (DK)

...etabolism, provides the basis for substrate-uncoupler-inhibitor titration (SUIT) protocols, and updates the terminology consistent with the MitoEAGLE white ...al-time_OXPHOS_analysis |Real-time OXPHOS analysis]]'''. — Richard Altmann’s bioblasts are the systematic unit of bioenergetics and chemiosmotic couplin

::::# [[MitoPedia: SUIT]] ...diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer. As a result of a sedentary life style and pr

...r EM, Shah DP, Philp AM, Hargett SR, Lawrence RT, Lee B, Sligar J, Carrive P, Tucker SP, Philp A, Lackner C, Turner N, Cooney GJ, Santos WL, Hoehn KL (2 ...d mitochondrial respiration in platelets: A future biomarker for Alzheimer’s disease?'''<br>

* Cecatto C, Cardoso LHD, Ozola M, Korzh S, Zvejniece L, Gukalova B, Doerrier C, Dambrova M, Gnaiger E, Makrecka-Kuka ...ts, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.

::::* 5 stars _ Hotel Bagués: ...n of mitochondrial capacities and create a Library of protocols: reference SUIT protocols, standard experimental media, and detailed instructions for OXPHO

...buffer with pure O₂ to minimize diffusion limitations at low ''P''_O2 in permeabilized myofibers [48]. ...ycin A (AA), 5 mM ascorbate + 0.5 mM ''N'',''N'',''N''’,''N''’-tetramethyl-p-phenylenediamine (TMPD).

::::* Stahel WA (2021) New relevance and significance measures to replace p-values. PLOS ONE 16:e0252991. https://doi.org/10.1371/journal.pone.0252991. ...comparison of data from two different laboratories. {Jenny: Also, in Gargi's paper the Seahorse data and the O2k data are treated as separate but comple

16:10 '''Perkhofer S''', Lass-Flörl C (Department für Hygiene, Mikrobiologie und * [[Jansen-Duerr P|'''Jansen-Dürr Pidder''']], Institute for Biomedical Ageing Research,

...diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer (Murdoch 2013 JAMA). ...ological applications. Centers of excellence will emanate from the project’s working groups providing training programmes and diagnostic services.