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...protocols'''. [[Prespecified protocols |Prespecified]] and [[time-stamped protocols]] that are publicly deposited may help to save Millions of Euros that may o

::::# [[MitoFit protocols]] ::::## [[Harmonization protocols]] (Begley, Ioannidis 2015)

=== Mitochondrial Bioenergetics : Methods and Protocols === .../doi/10.1007/978-1-61779-382-0_3 Mitochondrial Bioenergetics : Methods and Protocols]

...k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] ...solved. Accuracy is improved by standard numerical corrections. Graphs and protocols are stored and printed ready for publication.

The SUIT-004 protocols are designed to provide a quick assessment of the linear coupling control ( == Specific SUIT protocols ==

...Substrate-uncoupler-inhibitor titration''' ('''SUIT''') [[MitoPedia: SUIT |protocols]] are used with [[mitochondrial preparations]] to study respiratory control ==SUIT protocols==

In events obtained from a selected [[DL-Protocols |protocol]], the entire sequence of consecutive events is defined with even »[[DL-Protocols#DL-Protocol_principles|Protocol events]]

== Compare SUIT protocols ==

::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,GM) == Compare SUIT protocols ==

...Such conditions must be carefully evaluated, may require modifications of protocols, and must be corrected for acid-base reactions unrelated to glycolytic flux ...Such conditions must be carefully evaluated, may require modifications of protocols, and need data analysis beyond reporting changes of pH.

...overcome, providing a rationale for establishing a set of basic reference protocols. ...nce Camp 2016]]: Optimizing strategies on the malate concentration in SUIT protocols.

::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N(PM) == Compare SUIT protocols ==

== Specific SUIT protocols == == Compare SUIT protocols ==

== Specific SUIT protocols == :::+ Harmonization with many SUIT protocols (up to step 7S).

...DatLab when recording the data with the O2k. Usng [[MitoPedia: SUIT |SUIT protocols]], a sequence of respiratory steady-states is measured, marks are set, and ...'': The default [[data recording interval]] is 2 s, applied with most SUIT protocols. The larger the data recording interval, the lower is the time resolution a

::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,PGM) == Compare SUIT protocols ==

::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNS(Oct,PGM) :::+ Harmonization with many SUIT protocols (up to step 7S).

== Specific SUIT protocols == == Compare SUIT protocols ==

...Both methods are adaptable for compound screening; at the same time, these protocols provide easy-to-use and time- saving methods for ''in vitro'' studies of ce

...opose potential solutions for these problems, including '''improvements in protocols and documentation''', consideration of evidence from studies in progress, ' ::::* Make publicly available the full protocols, analysis plans or sequence of analytical choices, and raw data for all des

...control in high-resolution respirometry, aimed at standardization of SUIT protocols and fostering proficiency to generate experimental data of assured high qua ...using standardized mitochondrial reference samples, DatLab analysis, SUIT protocols and experimental design, normalization of flux and data reporting, O2k-Fluo

...provement over time. Ongoing quality assessment helps laboratories improve protocols and performance on a real-time basis to ensure optimal cryopreservation of

::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,GM) == Compare SUIT protocols ==

== Specific SUIT protocols == == Compare SUIT protocols ==

...lign: top;"> [[File:Set_upper_O2_limit_in_menu_bar.png|thumb|none|220px|'''Protocols menu''']] </li> <!--[[File:Set_lower_O2_limit_in_menu_bar.png|thumb|200px|'''Protocols menu''']]

== Specific SUIT protocols == == Compare SUIT protocols ==

...s, the present authors decided to investigate whether recurrent concussion protocols alter depressive-like phenotype behavior, and whether mitochondria play an

=== Protocols === ::::» [[Instrumental: Browse DL-Protocols and templates]]

:::: '''[[MitoPedia: SUIT]] - first steps towards a data bank of SUIT protocols''' :::: '''Updated terminology with SUIT protocols - open for discussion:''' [[Lemieux 2017 bioRxiv 103457 |Lemieux H, Blier P

Substrate-uncoupler-inhibitor-titration (SUIT) protocols are a combination of different substrates, inhibitors and uncouplers to ass In this outline two different SUIT protocols are described analyzing the difference in LEAK respiration measured with di

The SUIT-012 protocols specifically focus on assessing the coupling control ([[LEAK respiration|'' == Specific SUIT protocols ==

Numerous and different protocols have been developed or adapted to this subject. One However, the usual protocols using cuvette photometers can be both time consuming and

== Specific SUIT protocols == == Compare SUIT protocols ==

::::* Introduction to SUIT protocols for simultaneous measurement of O2 flux and H₂O₂ flux :::::* Substrate-uncoupler-inhibitor titration (SUIT) protocols </div> </div>

::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N(PGM) == Compare SUIT protocols ==

::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(PalOct,PGM) == Compare SUIT protocols ==

...S analysis with respiratory substrate-uncoupler-inhibitor titration (SUIT) protocols when working with isolated mitochondria, permeabilized cells or tissue homo

...etermine N-pathway [[Oxidative phosphorylation| ''P'']] capacity. SUIT-014 protocols provide an assessment of the coupling control [[LEAK respiration|''L'']]-[[ == Specific SUIT protocols ==

== O2k-Core manual and selected protocols ==

...mples is different between protocols and between the groups adopting these protocols. ...for an open discussion about these approaches in order to standardize the protocols for better reliability and reproducibility.

...A copy number in pluripotent and differentiating cells using real-time PCR protocols; assess expression of the mtDNA specific replication factors through real-t

...be examined using reference substrate-uncoupler-inhibitor titration (SUIT) protocols, and evaluated conditions for cell storage for later and reproducible respi

...lign: top;"> [[File:Set_lower_O2_limit_in_menu_bar.png|thumb|none|220px|'''Protocols menu''']] </li> <!--[[File:Set_lower_O2_limit_in_menu_bar.png|thumb|200px|'''Protocols menu''']]

::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(PGM) == Compare SUIT protocols ==

::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,PGM) == Compare SUIT protocols ==

== Specific DLP protocols == ...nd [[SUIT 003 AmR ce D059]] with carrier titration as a control. These two protocols have to be done in parallel.

::::# Titration vol. [µL]: In SUIT protocols, the value is entered as the volume of a titration, for calculating the dil

== Compare SUIT protocols ==

..., to address specific research interests, samples available, selected SUIT protocols, for further discussion with the Oroboros tutors. :::::» [[MitoPedia: SUIT|Library of SUIT protocols]]

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown during the training. The [[Gnaiger 2020 BEC MitoPathways| ...xtremely helpful and provide much guide and information regarding the O2k, protocols and bioenergetics.

..., to address specific research interests, samples available, selected SUIT protocols, for further discussion with the Oroboros tutors. :::::» [[MitoPedia: SUIT|Library of SUIT protocols]]

::::* Benefit from the unique features of [[DatLab |DatLab 7]] with [[SUIT protocols]]

...-Analysis templates are made available for specific [[MitoPedia: SUIT|SUIT protocols]], to express respiration as [[oxygen flux]] or [[oxygen flow]] normalized ...emplate for your specific SUIT protocol. In DatLab, in the pull-down menu 'Protocols: SUIT: Browse DL-Protocos and templates', select your specific SUIT protoco

...sions and new, user-friendly features on [[Run DL-Protocol/Set O2 limit|DL-protocols]], [[Export DL-Protocol User (*.DLPU)|DL-Protocol User]], quality control, ...ds= DatLab, [[MiPNet22.16 DatLab 7 Innovations: DL-Protocols|Innovation DL-Protocols]]

...measured, in WS and NWS, using two substrate-uncoupler-inhibitor titration protocols, at baseline (day 0), day 3 and 10 post-wounding, in STZ-DM and non-diabeti

...month interventions. Mitochondrial respiration was measured using two SUIT protocols in permeabilized muscle fiber bundles. Data were analyzed using a per-proto One hundred participants completed the ''a priori'' defined intervention protocols: (EX, ''N''=44; CON, ''N''=56). Weight loss was similar for both groups (Po

...is involved in redox homeostasis, particularly in the brain. In all, these protocols allow a detailed characterization of mitochondrial function in human neural

...of titration volumes and automatic control of complex titration-injection protocols. The TIP2k was specially developed for the O2k. The main modes of operation ::* Examples of proper TIP2k use and cleaning protocols

::::* Particularly the DL-Protocols may be of great interest to the expert O2k-users: ::::::» [[DL-Protocols]]

...n and controversial interpretation (Chacko et al 2014). Respirometric SUIT protocols for OXPHOS analysis (Sjoevall et al 2010, 2013, Pecina et al 2014) need to

...(95% CI: 0.928, 0.982), P < 0.001. Both the original and modified THRIFTY protocols generated type I and type II fibre pools of absolute purity. Using THRIFTY,

...|Bioblast pdf]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols contents]]

...mmercial- and working-'''solutions''' for improved consistency in relevant protocols. * In various protocols described on the Bioblast wiki, clarification and updates are required to p

:::#:Depending on whether or not you use [[Run_DL-Protocol/Set_O2_limit | DL-protocols]], chose:

...possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems. ...various combinations to provide links to the best [[MitoPedia: SUIT |SUIT protocols]]:

== Compare SUIT protocols == :::* [[SUIT-018]] are short protocols to study the oxygen dependence of O₂ flux and H₂O<sub

The SUIT-011 protocols are designed to study physiologically relevant maximum mitochondrial respir == Specific SUIT protocols ==

...for application of complex substrate-uncoupler-inhibitor titration (SUIT) protocols which are the basis for investigating mitochondrial pathways and respirator

...sts and advanced diagnostic substrate-uncoupler-inhibitor titration (SUIT) protocols within a single assay for exploring mitochondrial function.

...ysis, we used two substrate-uncoupler-inhibitor titration (SUIT) reference protocols, RP1 and RP2, interrogating 20 respiratory states. These include fatty acid ...cts, offering a more sensitive diagnostic approach compared to respiratory protocols with non-permeabilized living cells.

...a Sumbalova]], [[Krumschnabel G |Gerhard Krumschnabel]]: Towards reference protocols. * Standard protocols for blood cell separation, cell type characterization (purity, composition)

...ototype test scenario and expanding the range of application-specific SUIT protocols in the MitoFit-data base. O2k-Core applications are extended by application

...membrane potential). Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi

...nd [[SUIT-003 AmR ce D059]] with carrier titration as a control. These two protocols should be done in parallel. == Compare SUIT protocols ==

...tate]]s. A [[MitoPedia:_SUIT|library]] of evaluated and tested standard DL-Protocols is provided by the Oroboros team. The Titration-Injection-microPump [[TIP2k <div id="DL-Protocols-anchor1"></div>

...peroxide production. Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi

...ed out. Even so, we quite obviously lack unequivocal terminology, standard protocols, and perhaps most surprisingly, a common conceptual basis when performing s

*'Specific protocols and procedures for cells and isolated mito.' *'The protocols and the specifications.'

...[Oroboros O2k-technology]]. Substrate-uncoupler-inhibitor titration (SUIT) protocols allow the interrogation of numerous mitochondrial pathway and coupling stat

...iminish these conditions, but some high intensity interval training (HIIT) protocols have shown similar improvements in insulin sensitivity, in spite of the red

...endence only on limited amount of canonical substrates. Furthermore, these protocols can be very helpful for identifying substrates of other mitochondrial metab :::: During my STSM , I will be focusing on development of protocols for permeabilized cells of immortalized murine brown adipocyte line (BMC).

...mption in real-time with high sensitivity in various tissues and substrate protocols. While most of the studies rely on saturating concentrations of substrates

...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and is ...pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]]

S(Rot) pathway in the LEAK state can be evaluated in the following SUIT protocols: S(Rot) pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

== Compare SUIT protocols == ::::* [[SUIT-008]] 1PM;2D;3G;4S;5U;6Rot-: SUIT-008 protocols are designed to assess the additivity between the [[NADH_Electron_transfer-

...ls, based on the latest experience with [[Pesta_2012_Methods_Mol_Biol|SUIT protocols]] developed for functional mitochondrial diagnosis. Catherine trained Austi ...e achieved by continued instrumental development, advancing and testing of protocols for functional mitochondrial diagnosis, and international cooperation. A sp

...h weight per experimental run, supporting application of complex titration protocols.</div>

...iable data in commonly used substrate-uncoupler-inhibitor-titration (SUIT) protocols and cyanide titration is needed to obtain precise data for COX excess capac ...hway and coupling control on mitochondrial ''p''₅₀. During SUIT protocols, kinetic data for oxygen was obtained with aerobic-anaerobic transitions in

=== SUIT protocols for high-resolution respirometry === ::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.

...n on 96-well SPA plates monitored noninvasively in real time. (2) Numerous protocols designed for the measurement of cellular metabolism use ¹⁴C-labe

...nson's, and Huntington's diseases. Here we provide detailed guidelines and protocols as standardised across the five collaborating laboratories of the CeBioND c

:::: '''Standard operating procedures and user requirement document: Protocols, terminology, documentation''' ::::» [[WG1 MitoEAGLE protocols, terminology, documentation |WG1 Action]]

...Mitchell T, Darley-Usmar VM (2014) Bioenergetics and the oxidative burst: protocols for the isolation and evaluation of human leukocytes and platelets. J Vis E

...oupler-inhibitor titration|respiratory flux at various steady-states, SUIT protocols]]. ...omplemented by lectures on mitochondrial physiology (general topics; [[O2k-Protocols]]). Short presentations of 'O2k perspectives' provide participants with the

...and substrate control states [1] were established in 6 different [[SUIT]] protocols. In calculation of ΔΨ, mitochondrial protein was normalized to citrate sy |keywords=ΔΨ, Mitochondrial preparations, [[SUIT]] protocols

| Discussion about biological samples, experimental design, SUIT protocols <br>

...gh-resolution respirometry of intact human peripheral blood cells. Several protocols were used – with and without rotenone inhibition of CI in the screening c ...e increase in oxygen consumption was ruled out using specific respirometry protocols and flow cytometry (assessing mitochondrial membrane potential using the pr

...atio]] (RCR) [6,7]. Applicaton of [[CI+II]] substrate combinations in SUIT protocols extends conventional bioenergetic studies to the level of mitochondrial phy ...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

::::::* SUIT-Protocols » [[Run DL-Protocol/Set O2 limit]] ::::* Benefit from the unique features of [[DatLab |DatLab 7]] with [[SUIT protocols]]

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.

...y contribute to the development of accurate chemotherapeutic sensitization protocols. ...allow for the development of more accurate chemotherapeutic sensitization protocols.

...or ''in situ'' mitochondrial functional analysis using digitonin. Standard protocols were used for immunoblotting and proteins were separated on denaturing gels

|title=Optimizing strategies on the malate concentration in SUIT protocols. |abstract=Substrate-uncoupler-inhibitor-titration (SUIT) protocols used in high-resolution respirometry have been developed [1] to evaluate th

(2) Harmonization of experimental procedures (protocols) is a basic requirement to increase the robustness of information and knowl ...s to research targeted at hypothesis testing. Experimental optimization of protocols including negative results should be made publicly available at an early ti

...oboros O2k-FluoRespirometry, Innsbruck, Austria) and reference respiratory protocols.

=== SUIT protocols for high-resolution respirometry === :::: Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or obta ...s capacity, residual oxygen consumption, and, by extension of conventional protocols, intactness of cell membranes. In mitochondrial preparations such as isolat

|title=Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscl [[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]]

...common cross-linked respiratory states, allowing for harmonization of both protocols. The fluxes were corrected for the contribution by contaminating cells.

...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibres from small biopsies of muscle, and is == HRR: high-resolution respirometry and SUIT protocols ==

...mnSysProtMenu0 already exists' appears when trying to load instrumental DL-protocols using [[DatLab]]. ::::## When loading a new instrumental DL-Protocol ('Protocols' > 'Run DL-Protocol / Set O2 Limit'), click on SUIT instead;

...s, living cells). Optimized substrate-uncoupler-inhibitor titration (SUIT) protocols offer a powerful tool for comprehensive mitochondrial physiology analysis b ...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and is

...membrane potential). Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi

...espirometry with the calibration routine using instrumental calibration DL-Protocols in [[DatLab]]. == Instrumental DL-Protocols ==

...sented. Furthermore, this workshop covers interpretation of data from SUIT protocols.

...eful to see the SUIT protocols, the POS services, the background and other protocols related to the O2K. In my opinion, it was a great experience.'' ...peroxide production. Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi

...tion respirometry using substrate-uncoupler-inhibitor titration ([[SUIT]]) protocols on permeabilized fibers [1,2]. The patient performed an in-vivo phosphorus- ...Mol. Biol.|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.

::::* Homogenizer-based protocols that require extensive operator experience (highly skilled personnel) to ob ::::* The current protocols rely on Dounce, Potter-Elvehjem, and rotor/stator shearing homogenization:

..., as it offers a large collection of relevant preprints, publications, and protocols, which are always up-to-date and therefore create a platform where recent k ...l in the process of final manuscript preparation, such as harmonization of protocols, which not only help to create clear guidelines for other researchers but a

...ilable components of QA''': methodological details, exchange of laboratory protocols with updates. ::2. '''Science sessions''': protocols, traces, results, concepts, perspectives.

...ture of biomedical and patient specimens with respect to experimental SUIT protocols to be applied.

...for 1 h at 37 °C. Different substrate-uncoupler-inhibitor titration (SUIT) protocols were used for diagnostic OXPHOS analysis. Using inhibitors of the main mito ...ave a role in WI during FAO. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H₂O₂

...n would be standardization of muscle stem cells bioenergetic determination protocols and mitochondria function data collection. As well, let me point out that w :::: (2) Standardized and updated protocols in muscle stem cell respiration/glycolysis measurement (XF Seahorse analyze

...mitochondrial capacities and create a Library of protocols: reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis ...k-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks for mitochon

...ce races (R1, R2). Muscle OXPHOS capacity was determined using 2 titration protocols without (SUIT 1) or with pyruvate (SUIT 2) as substrate. Electrons enter at

...rent methods in healthy human volunteers. In addition, we are applying the protocols to a porcine trauma brain injury model, to analyze mitochondrial (dys-)func ...e are obtained from healthy human volunteers. Further, we are applying our protocols to a porcine TBI model, which we are currently establishing in our laborato

...HC (n = 4 each) were performed. After optimising isolation and processing protocols for functional studies in PBMC, mitochondrial oxidative responses to substr

|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Procedures|O2k-Procedures contents]] High-resolutio

...-FluoRespirometer (Oroboros Instruments, Austria). Two harmonized [[SUIT]] protocols [[SUIT-004 O2 pfi D010|RP1]] and [[1OctM;2D;3P;4S;5U;6Rot-|RP2]] [1] modifi ...44). Rotenone-sensitive respiration was higher after the excercise in both protocols (RP1: p=0.025 and RP2: p=0.001). The increase in different mitochondrial re

...igh oxygen regime can be done using dithionite titrations following the DL-Protocols: Instrumental high O2 background_manual injections or Instrumental high O2 ...dium. All Instrumental DL-Protocols available can be found in C:\DatLab\DL-Protocols\Instrumental\ (default directory after installation of DatLab).

...by using different types of Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocols (SUIT1 and SUIT2) where we evaluated the possible deviations between differ ...unlimited titrations and, therefore, the possibility to apply various SUIT protocols designed specifically for a certain project.

...espiration was assessed by Oroboros Oxygraph-2k using harmonized reference protocols.

...ventional impact metrics. Preprints are not peer-reviewed, but reflect the time-stamped state of the author's work presented to the scientific community. Differenc ...ith a rigorous quality management, such as pre-publication of time stamped protocols, access to raw data of the complete data set, including calibration procedu

...ted anoxic transitions in each respiratory state in the course of [[SUIT]] protocols. ::::# Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...epilept(ogen)ic medication. Therefore, we are in the process of developing protocols to analyze different mitochondrial parameters using the Oxygraph-2K (Orobor

...ph-2k applying standard substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocols for permeabilized cells in [[MiR05]]. For HRR with intact cells, cyropreser

...models are the preferred choice for many researchers worldwide. Yet, study protocols for the induction of diet-induced obesity (DIO) in mice vary with respect t

...y''. Links will be made available to relevant websites, including selected protocols for practical approaches to MiP. In due time, a hard copy will be produced

::::# PC(DatLab[DL-Protocols{F<How to Find instructions in F, Add cells>}]) — '''''Found''''' !! ...[F{How to Find instructions in F, Add cells}]): In DatLab, the selected DL-Protocols designed for 'cells' as a sample type, include within the DL-Protocol the F

ex vivo. With different titration protocols, detailed information of the cellular

...eful to see the SUIT protocols, the POS services, the background and other protocols related to the O2K. In my opinion, it was a great experience. '' - [[Romero ...peroxide production. Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi

...states and applications of substrate-uncoupler-inhibitor titration (SUIT) protocols. - ''Last update: 2017-07-22'' :::: '''Session C''' (morning) '''OXPHOS protocols and data reporting'''

...location and phosphorylation (OXPHOS), mitochondrial pathways, respiratory protocols for diagnosis of mitochondrial function, mitochondrial respiratory control

...are shown in Figure 2. Analysis will focus on comparing results from both protocols. RP2 is designed to reveal the differences in fatty acid oxidation between

# Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscl

...prehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control. Mitochondrial pathways and coupling'''

...fferent incubation temperatures (38, 40, 42, and 44°C) using standard SUIT protocols. Effect of incubation temperature was analyzed using repeated measures one-

...d inhibition of ETC components. Under such conditions mSLP is addressed by protocols relying on membrane potential measurements using safranine O, also monitore

...working with "mitochondria people", getting to know the Oxygraph, methods, protocols. I am well certain that soon I will be also a user of the Oroboros instrume :::: '''A1''': Diagnostic substrate-uncoupler-inhibitor titration (SUIT) protocols of mitochondrial fitness.

In consequence, in addition to standardization of cell separation protocols and of experimental media, research on the stability of biological samples

...on FluoRespirometers. Using substrate-uncoupler-inhibitor titration (SUIT) protocols with various fuel substrate combinations in OXPHOS-, LEAK- and ET-states, w

:::: • Optimally harmonisation protocols across research groups :::: 1st part: arrival, becoming familiar with facilities, protocols, learning techniques (myocyte isolation, SPA and CO2 trapping assays) and d

...ub>O₂ production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H₂O₂

...on ice from 1 to 150 h. Substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocols were applied on permeabilized fibres using high-resolution respirometry ([[

# Protocols for high-resolution respirometry: focus on combined measurement of respirat # Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...'': The default [[data recording interval]] is 2 s, applied with most SUIT protocols. The larger the data recording interval, the lower is the time resolution a

...urately. Such details are subject to change, and an evolutionary series of protocols (with numbered versions) is maintained up-to-date. By reference to the vers

[[File:DLP-Cleaning_window.png|right|700px|'''Instrumental DL-Protocols for cleaning''']] ...'' [Instrumental:_Browse_DL-Protocols_and_templates Browse Instrumental DL-Protocols for cleaning]

...ls.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols contents]]Preparation of permeabilized muscle fibers for diagnosis of mitoc ...njunction with multiple [[substrate-uncoupler-inhibitor titration]] (SUIT) protocols, respirometric studies of human and animal tissue biopsies improve our fund

...Platforms', together support the preparation of effective data-collection protocols and provide a central reference to facilitate harmonization. The DataSHaPER ...le calibration and pooling of data that had been collected under different protocols in different centres, so that data synthesis was rendered valid.

...ck): '''Experimental validation of substrate-uncoupler-inhibitor titration protocols with NIH3T3 fibroblasts.''' ([[Pesta 2012 Methods Mol. Biol.|Abstract]]) ...Mol. Biol.|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...le:Koopman_MITOEAGLE_Barcelona_2017_Figure.png|left|450px |ETS and NS-SUIT protocols]]

# Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscl ...nto several O2k-chambers for technical repeats or application of different protocols, and subsamples can be stored away for other tests. These tests convinced u

The fully updated O2k-Manual, O2k-Protocols, and O2k-References (pdf reprints) will be available on '''your personal Or ...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...peroxide production. Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi

...|left|90px|Carolina Anneliese Doerrier]] Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers.

::* Lectures and discussion on protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membra

...might be added after stimulation of respiration by ADP in various [[SUIT]] protocols. Arguments against additions of ATP, however, need to be considered, e.g. a

{{Template:Cited by Huete-Ortega M 2021 MitoFit Photosynthesis protocols}} ::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

SUIT protocols from the [[SUIT-003]] family will guide you through the titrations needed t The DLPs for AmR [[SUIT]] protocols already come with multiple steps of H₂O₂ calibrations

...management network will be a strategic innovation to develop harmonization protocols towards generating a rigorously monitored data repository on mitochondrial ...y is required to implement quality control systems and optimally harmonize protocols across research groups. The primary challenge is to ‘increase value and r

...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and is

...' was performed in local anesthesia using Bergström needle technique. SUIT protocols RP1 and RP2 [4] were used to evaluate oxygen consumption rate (pmol s<sup>-

...issues. The active user forums and O2k-Network serve as a rich source of protocols, shared knowledge, and information on all topics related to bioenergetics a ...oros is Erich's groups outstanding efforts to provide detailed methods and protocols for both the machines and experiments... .'' - '''[[Hickey Anthony JR|Anth

::::* Design new protocols and instrumental tools to measure ''pmP''

...ed with DatLab7.4. For further information, see: [[Instrumental: Browse DL-Protocols and templates]] :::: The DLPs for AmR SUIT protocols already come with multiple steps of H₂O₂ calibrations

:::* WG1 '''[[MitoEAGLE protocols, terminology, documentation]]'''

::: [[MitoEAGLE protocols, terminology, documentation |WG1: MitoEAGLE protocols, terminology, documentation]] ::::* [[Doerrier 2018 MiP2018a |Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers]]

::::* Integration of the new protocols in the DatLab software ...nd correction, experimental SUIT (substrate-uncoupler-inhibitor-titration) protocols and data analysis.

[[File:SUIT-nomenclature.jpg|right|400px|link= MitoPedia: SUIT|SUIT protocols]] === A1: Diagnostic SUIT protocols of mitochondrial fitness ===

...-Fluorometry will be presented in overview lectures and hands-on sessions; protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membra

...ps://www.ncbi.nlm.nih.gov/pubmed/17406588 Frezza ''et al.'' (2007), Nature Protocols] ...Fighting against reproducibility crisis: Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers. - [[M

# Pesta D, Gnaiger E (2012) High-resolution respirometry: OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscl

::::#Pesta D, Gnaiger E (2012) High-resolution respirometry: OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscl

{'''SUIT protocols in mitochondrial research are designed to:''' || SUIT protocols aim to assess function, not to disrupt DNA.

...Maran et al 1997). These studies established a first level of bioenergetic protocols for biomedical applications of high-resolution respirometry. The scientific ...of the O2k-Fluorometer by the two companies, and development of diagnostic protocols for methodological evaluation of the instrument with muscle fibres, blood c

...sion injury [5-8]. These studies established a first level of bioenergetic protocols for biomedical applications of high-resolution respirometry [9-12]. With co

...whole blood sampling (''e.g.'', K-EDTA, lithium heparin, sodium citrate). Protocols are optimized on the basis of yield, cell fraction purity, intactness of pl :::::# Specific protocols are used for respiration assessments in living and permeabilized. The type

::::*standard protocols/methodologies lecture ::::*protocols (why use specific method or reagent)

| White adipose tissue mitochondrial respiration protocols - Experiments and Results discussion | Inter-laboratory harmonization of protocols to set a final consensus for the evaluation of the technical skills needed

...Such conditions must be carefully evaluated, may require modifications of protocols, and need data analysis beyond reporting changes of pH.

::::## Quality control: instrumental and methodological details, experimental protocols

...ically revealed by the respirometric approach to OXPHOS analysis with SUIT protocols.

:::: When membrane potentials obtained by different protocols but using the same parameters (binding correction factors) during calculati ...s for membrane potentials of the same state obtained via totally identical protocols are to be compared between different samples a correction may not be strict

...ium will collaborate on the implementation of refined and novel diagnostic protocols as a milestone towards an internationally recognized center of excellence, ...nd particularly in Tyrol. Comprehensive mitochondrial and exercise testing protocols will be developed and validated in these studies. Case studies will transla

# Kern H, Hofer C, Mayr W, Carraro U (2009) European Project RISE: Partners, protocols, demography. Basic Appl Myol/ Europ J Translat Myol 19: 211-216.

The following SUIT protocols are specifically optimized for PBMC and platelets <br> ...ons of mitochondrial pathways''' in health and disease with SUIT reference protocols. <br>

...issues. The active user forums and O2k-Network serve as a rich source of protocols, shared knowledge, and information on all topics related to bioenergetics a ...e across your website and found excellent information for instrumentation, protocols and solutions.'' - '''[[Dworetzky S|Steven Dworetzky]]''', Knopp Neuroscie

...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...LE) in studies of mitochondrial function. MitoEAGLE develops harmonisation protocols towards generating a data repository on mitochondrial respiratory function. ...mitochondrial capacities and create a Library of protocols: reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis

Image:IOC106 (15).jpg|Discussions with Erich about protocols.

...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...uncoupler-inhibitor titration|substrate-uncoupler-inhibitor titration SUIT protocols]]) and experiments run in "Special interest groups". Short presentations of

...rdoso''']], [[Timon-Gomez Alba |'''Alba Timon-Gomez''']]: Let's talk about protocols to measure mitochondrial respiratory function - an introduction to OXPHOS a

...thor contributions and acknowledgements. Taken together, '''the 'Vancouver protocols' are used as guidelines for conducting [[research]] and open communication === BenchTalk - sharing of protocols, methods, and techniques ===

|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] ::::» [[O2k-Protocols]]

7. Protocols :* Present and discuss details of experimental projects, protocols, data analysis and interpretation

|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols]] :>> [[O2k-Protocols]]

...itochondria. … The degree of mitochondrial integrity obtained with current protocols has been questioned,31 with less disruptive approaches involving permeabili ...rst, we transitioned from tissue mitochondrial isolation techniques to new protocols developed to utilize tissue homogenates for mitochondrial respiration. Homo

...'''[[Granata Cesare|Cesare Granata]]''': ''Novel mitochondrial respiration protocols reveal organ-specific reliance on ketone body metabolism in mice.''

...ism, provides the basis for substrate-uncoupler-inhibitor titration (SUIT) protocols, and updates the terminology consistent with the MitoEAGLE white paper on M ...lect physiological respiratory states in mitochondrial preparations. These protocols ignored the importance of cataplerotic metabolite depletion in the tricarbo

|'''Protocols that SUIT your research question'''<br> Using '''S'''ubstrate-'''U'''ncoupler-'''I'''nhibitor-'''T'''itration protocols you can study mitochondrial respiratory control through a sequence of coupl

...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

|''' What are the substrate-uncoupler-inhibitor titration reference protocols?'''<br> These protocols provide a common baseline for comparison of mitochondrial respiratory contr

The following SUIT protocols are specifically optimized for PBMC and platelets <br> The DLPs for AmR [[SUIT]] protocols already come with multiple steps of H₂O₂ calibrations

See how to modify existing DL-protocols. |'''Are you a beginner or need a refresher on using SUIT protocols with DatLab7.4? '''<br>

...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibres from small biopsies of muscle, and is :::# Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl